ArticleMethods in molecular biology (Clifton, N.J.)2024
Sequencing of Transcriptome-Wide Poly(A) Tails by PAIso-seq.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Mining, validating, and quantifying circular RNA transcriptome from total RNA as a biomarker or target.Scientific reports · 2025Article
- Maternal mRNA deadenylation is defective in in vitro matured mouse and human oocytes.Nature communications · 2024Article
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Authors and funding
3 authors.
Funding
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Abstract
Poly(A) tails are added to most eukaryotic mRNA and have essential regulatory functions. However, due to its homopolymeric nature, the sequence information in poly(A) tails is challenging to obtain in transcriptome measurement studies. In this chapter, we describe the detailed procedures of poly(A) inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.
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