RNA-Binding Protein-Mediated mRNA Deadenylation in Mammalian Cell Extracts.

Wi S Lai, Stephanie N Hicks, Perry J Blackshear

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 54% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Wi S LaiSignal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.

Stephanie N HicksSignal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.

Perry J BlackshearSignal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. black009@niehs.nih.gov.

National Institute of Environmental Health Sciences · USDuke Medical Center · US

Funding

TTP and related proteins in the regulation of gene expressionZIAES090080 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI BLACKSHEAR, PERRY J · 2009 to 2025

$52.6M

Intramural NIH HHS ZIA ES090080

6 · The paper itself

Abstract

Removal of the poly(A) tail, or deadenylation, is a crucial step in destabilizing mRNAs in eukaryotes. In this chapter, we describe a cell-free deadenylation assay that uses cytoplasmic cell extracts from human HEK293 cells transiently transfected with DNA encoding RNA-binding proteins (RBP), and in vitro-transcribed, radiolabeled, RNA probes. We include methods to evaluate the effects of RBPs or deadenylases on various in vitro-transcribed probes, with or without poly(A) tails. Finally, we also demonstrate the adaptability of these assays to test purified protein components in our cell-free deadenylation assay. In our experience, these methods are well suited for the initial assessment of the effects of RBPs on the deadenylation of mRNAs.

Indexed as

RNARNA-Binding ProteinsAnimalsCell ExtractsHEK293 CellsHumansMammalsPoly ARNA, MessengerRNA StabilityCell ExtractsPoly ARNARNA-Binding ProteinsRNA, MessengerCapped RNA probesCytosolic extractsDeadenylationmRNA-binding proteins (RBPs)Poly(A)

Identifiers

PMID37824071

DOI10.1007/978-1-0716-3481-3_11

PMCPMC11025660

OpenAlexW4387562938

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.