Evidence map›Paper›PMID 37823951›Full record

ArticleIrish journal of medical science2024

MicroRNA-582-3p knockdown alleviates non-alcoholic steatohepatitis by altering the gut microbiota composition and moderating TMBIM1.

Shuo Huang, Xia Xiao, Hongman Wu, Feng Zhou, Chenchao Fu

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Article in Irish journal of medical science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
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7citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  7. Molecular Morbidity Score-Can MicroRNAs Assess the Burden of Disease?International journal of molecular sciences · 2024
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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Shuo HuangDepartment of Internal Medicine, Hunan Maternal and Child Health Hospital, Changsha, Hunan, 410013, China.
Xia XiaoDepartment of Internal Medicine, Hunan Maternal and Child Health Hospital, Changsha, Hunan, 410013, China.
Hongman WuDepartment of Infection Control Center, Xiangya Hospital of Central South University, NO.87, Xiangya Road, Changsha, Hunan, 410008, China.
Feng ZhouDepartment of Infection Control Center, Xiangya Hospital of Central South University, NO.87, Xiangya Road, Changsha, Hunan, 410008, China.
Chenchao FuDepartment of Infection Control Center, Xiangya Hospital of Central South University, NO.87, Xiangya Road, Changsha, Hunan, 410008, China. Fuchenchao6112@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe gut dysbiosis correlates with non-alcoholic steatohepatitis (NASH), involving the moderation of miRNAs.

aimsThis study was aimed to investigate the correlation between gut microbiota and miR-582-3p in patients with non-alcoholic steatohepatitis (NASH) and to explore the possible regulation of miR-582-3p in the function of the activated hepatic stellate cells (HSCs).

methodsGSE69670 and GSE14435 datasets were analyzed by GEO2R. Plasma and fecal samples were obtained from the subjects, non-steatosis (n = 35), simple steatosis (n = 35), and NASH (n = 35). The variations in intestinal microbiota in the non-steatosis and NASH groups were analyzed using 16S rRNA sequencing. The expression of miR-582-3p among the groups was detected using RT-qPCR. Correlations between top-changed intestinal microbiota and miR-582-3p expression were analyzed using the Pearson correlation coefficient. Target gene identification was performed by prediction and dual-luciferase reporter assay. The effect of miR-582-3p on the cell function of TGF-β1-induced HSCs was assessed in vitro.

resultsmiR-582-3p was the common differentially expressed miRNA between GSE69670 and GSE14435. miR-582-3p was upregulated in NASH patients' plasma, as well as in TGF-β1-induced LX-2 cells. The non-steatosis and NASH groups showed significantly different intestinal microbiota distribution. miR-582-3p was positively correlated with specific microbiota populations. TMBIM1 was a target gene for miR-582-3p. Knockdown of miR-582-3p suppressed HSC proliferation and myofibroblast markers' expression but induced cell apoptosis, via TMBIM1.

conclusionsThis present study suggests that miR-582-3p promotes the progression of NASH. Knockdown of miR-582-3p may alleviate NASH by altering the gut microbiota composition and moderating TMBIM1.

Indexed as

Gastrointestinal MicrobiomeMembrane ProteinsMicroRNAsNon-alcoholic Fatty Liver DiseaseHumansRNA, Ribosomal, 16STransforming Growth Factor beta1Membrane ProteinsMicroRNAsMIRN582 microRNA, humanRECS1 protein, mouseRNA, Ribosomal, 16STransforming Growth Factor beta1Gut microbiotaHepatic stellate cellMicroRNA-582-3pNon-alcoholic steatohepatitis

Identifiers

PMID37823951

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.