Evidence map›Paper›PMID 37823268›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2023

Single-Molecule Spatial Transcriptomics of Human Thoracic Aortic Aneurysms Uncovers Calcification-Related

Dogukan Mizrak, Yang Zhao, Hao Feng, Jane Macaulay, Ying Tang, Zain Sultan, Guizhen Zhao, Yanhong Guo, Jifeng Zhang, Bo Yang and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 13 citations in OpenAlex.

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  13. Editorial: Single-cell OMICs analyses in cardiovascular diseases.Frontiers in cardiovascular medicine · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Dogukan Mizrak *Department of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-9885-9686
Yang Zhao *Department of Internal Medicine (Y.Z., G.Z., Y.G., J.Z., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-1428-5145
Hao FengDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.
Jane MacaulayDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.
Ying TangDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.
Zain SultanDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-6790-8334
Guizhen ZhaoDepartment of Internal Medicine (Y.Z., G.Z., Y.G., J.Z., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-2648-8986
Yanhong GuoDepartment of Internal Medicine (Y.Z., G.Z., Y.G., J.Z., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-3334-9159
Jifeng ZhangDepartment of Internal Medicine (Y.Z., G.Z., Y.G., J.Z., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0001-5161-4705
Bo YangDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0002-2158-9155
Y Eugene ChenDepartment of Cardiac Surgery (D.M., H.F., J.M., Y.T., Z.S., B.Y., Y.E.C.), University of Michigan Medical Center, Ann Arbor.ORCID 0000-0003-2357-7825
University of Michigan · USZunyi Medical University · CN

Funding

Using genetics to discover mechanisms of myocardial infarctionR01HL109946 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YUQING Eugene CHEN, Ida Liisa Surakka · 2011 to 2026
$8.9M
KLF14 and Cardiovascular DiseaseR01HL134569 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE · 2017 to 2024
$5.3M
Therapeutic Insights into Loeys-Dietz Syndrome using Humanized ModelsR01HL151776 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Bo Yang · 2020 to 2026
$4.2M
Define the mechanisms of aortopathy in bicuspid aortic valve patientsR01HL141891 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YANG, BO · 2018 to 2022
$3.5M
Development of phospholipid-based nanotherapeutics for treating abdominal aortic aneurysmR01HL165688 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Yanhong Guo, Anna Schwendeman · 2023 to 2026
$3.1M
Nitro-Fatty Acids and Cardiovascular DiseaseR01HL162294 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE, GARCIA-BARRIO, MINERVA T · 2022 to 2025
$2.9M
Vascular smooth muscle cell ferroptosis and abdominal aortic aneurysmR01HL166203 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Yanhong Guo · 2023 to 2026
$2.7M
BAF60c and abdominal aortic aneurysmR01HL153710 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, JIFENG · 2021 to 2025
$2.7M
Browning of perivascular adipose tissue protects against thoracic aortic aneurysmR01HL159871 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CHEN, YUQING EUGENE · 2022 to 2025
$2.3M
Effect of mutations in the TGF-beta pathway genes on smooth muscle cell differentiation using patient derived iPSCs.K08HL130614 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YANG, BO · 2016 to 2020
$848k
NHLBI NIH HHS K08 HL130614NHLBI NIH HHS R01 HL109946NHLBI NIH HHS R01 HL134569NHLBI NIH HHS R01 HL141891NHLBI NIH HHS R01 HL151776NHLBI NIH HHS R01 HL153710NHLBI NIH HHS R01 HL159871NHLBI NIH HHS R01 HL162294NHLBI NIH HHS R01 HL165688NHLBI NIH HHS R01 HL166203
6 · The paper itself

Abstract

backgroundAlthough single-cell RNA-sequencing is commonly applied to dissect the heterogeneity in human tissues, it involves the preparation of single-cell suspensions via cell dissociation, causing loss of spatial information. In this study, we employed high-resolution single-cell transcriptome imaging to reveal rare smooth muscle cell (SMC) types in human thoracic aortic aneurysm (TAA) tissue samples.

methodsSingle-molecule spatial distribution of transcripts from 140 genes was analyzed in fresh-frozen human TAA samples with region and sex-matched controls. In vitro studies and tissue staining were performed to examine human CART prepropeptide (

resultsWe captured thousands of cells per sample including a spatially distinct

conclusionsHere, we demonstrate the feasibility of single-molecule imaging in uncovering rare SMC subtypes in the diseased human aorta, a difficult tissue to dissociate. We identified a spatially distinct

Indexed as

Aortic Aneurysm, ThoracicCalcinosisAorta, ThoracicGene Expression ProfilingHumansMaleMyocytes, Smooth MuscleTranscriptomeaortaaortic aneurysm, thoracicmyocytes, smooth musclesingle-cell gene expression analysis

Identifiers

PMID37823268
PMCPMC10842613
OpenAlexW4387563383

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.