ArticleThe Journal of cell biology1986
Contractile activity is required for the expression of neonatal myosin heavy chain in embryonic chick pectoral muscle cultures.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 76 citations in OpenAlex.
- In vitro Differentiation of Functional Human Skeletal Myotubes in a Defined System.Biomaterials science · 2014Article
- Acute heat stress prior to downhill running may enhance skeletal muscle remodeling.Cell stress & chaperones · 2012Article
- Myosin heavy chain mRNA isoforms are expressed in two distinct cohorts during C2C12 myogenesis.Journal of muscle research and cell motility · 2012Article
- Regulation of myosin heavy chain expression during rat skeletal muscle development in vitro.Molecular biology of the cell · 2001Article
- Spontaneous activity regulates calcium-dependent K+ current expression in developing ascidian muscle.The Journal of physiology · 1998Article
- Co-ordinated modulation of Ca2+ and K+ currents during ascidian muscle development.The Journal of physiology · 1996Article
- Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.The Journal of cell biology · 1996Article
- Motility of bile canaliculi in the living animal: implications for bile flow.The Journal of cell biology · 1991Article
- Regulation of protein turnover in skeletal and cardiac muscle.The Biochemical journal · 1991Review
- Long-term maintenance of primary myogenic cultures on a reconstituted basement membrane.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Article
- Myogenic programs of mouse muscle cell lines: expression of myosin heavy chain isoforms, MyoD1, and myogenin.The Journal of cell biology · 1990Article
- Differential distribution of myosin isoforms among the myofibrils of individual developing muscle fibers.The Journal of cell biology · 1990Article
- Myogenesis and histogenesis of skeletal muscle on flexible membranes in vitro.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Article
- Expression and DNA sequence analysis of a human embryonic skeletal muscle myosin heavy chain gene.Nucleic acids research · 1989Article
- Distribution of fast myosin heavy chain isoforms in thick filaments of developing chicken pectoral muscle.The Journal of cell biology · 1989Article
- Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation.The Journal of cell biology · 1988Article
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2 authors.
Funding
Abstract
The expression of neonatal myosin heavy chain (MHC) was examined in developing embryonic chicken muscle cultures using a monoclonal antibody (2E9) that has been shown to be specific for that isoform (Bandman, E., 1985, Science (Wash. DC), 227: 780-782). After 1 wk in vitro some myotubes could be stained with the antibody, and the number of cells that reacted with 2E9 increased with time in culture. All myotubes always stained with a second monoclonal antibody that reacted with all MHC isoforms (AG19) or with a third monoclonal antibody that reacted with the embryonic but not the neonatal MHC (EB165). Quantitation by ELISA of an extract from 2-wk cultures demonstrated that the neonatal MHC represented between 10 and 15% of the total myosin. The appearance of the neonatal isoform was inhibited by switching young cultures to medium with a higher [K+] which has been shown to block spontaneous contractions of myotubes in culture. Furthermore, if mature cultures that reacted with the neonatal antibody were placed into high [K+] medium, neonatal MHC disappeared from virtually all myotubes within 3 d. The effect of high [K+] medium was reversible. When cultures maintained in high [K+] medium for 2 wk were placed in standard medium, which permitted the resumption of contractile activity, within 24 h cells began to react with the neonatal specific antibody, and by 72 h many myotubes were strongly positive. Since similar results were also obtained by inhibiting spontaneous contractions with tetrodotoxin, we suggest that the development of contractile activity is not only associated with the maturation of myotubes in culture, but may also be the signal that induces the expression of the neonatal MHC.
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