ArticleThe Journal of cell biology1986
Monoclonal antibody specific for human nuclear proteins IEF 8Z30 and 8Z31 accumulates in the nucleus a few hours after cytoplasmic microinjection of cells expressing these proteins.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Cerebellar Purkinje cells incorporate immunoglobulins and immunotoxins in vitro: implications for human neurological disease and immunotherapeutics.Journal of neuroinflammation · 2009Article
- Lamin A/C is a risk biomarker in colorectal cancer.PloS one · 2008Article
- Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes.Molecular biology of the cell · 1999Article
- Receptor-mediated cellular entry of nuclear localizing anti-DNA antibodies via myosin 1.The Journal of clinical investigation · 1997Article
- Nuclear localization signals also mediate the outward movement of proteins from the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Nucleocytoplasmic transport.The Biochemical journal · 1994Review
- Molecular and structural analysis of nuclear localizing anti-DNA lupus antibodies.Immunologic research · 1994Article
- Nucleocytoplasmic shuttling of the progesterone receptor.The EMBO journal · 1991Article
- A nuclear localization signal binding protein in the nucleolus.The Journal of cell biology · 1990Article
- Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.The Journal of cell biology · 1990Article
- Antiviral state against influenza virus neutralized by microinjection of antibodies to interferon-induced Mx proteins.The EMBO journal · 1988Article
- Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells.The Journal of cell biology · 1987Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A monoclonal antibody (mAB 1C4C10) that reacts specifically with human nuclear proteins IEF 8Z30 and 8Z31 (charge variants; HeLa protein catalogue number; Bravo, R., and J. E. Celis, 1982, Clin. Chem., 28:766-781) has been microinjected into the cytoplasm of cultured cells that either express (primates) or lack these proteins (at least having similar molecular weights and pIs; other species), and its cellular localization has been determined by indirect immunofluorescence. Nuclear localization (nucleolar and nucleoplasmic) of the antibody was observed only in cells expressing these antigens, suggesting that a determinant present in IEF 8Z30 and 8Z31 is required for cytoplasm-nuclear translocation. Nuclear migration was not inhibited by cycloheximide, implying that these proteins may shuttle between nucleus and cytoplasm. The results assumed to support the signal rather than the free diffusion model are further supported by microinjection experiments using antibodies (proliferating cell nuclear antigen/cyclin, DNA) that react with nuclear components but do not recognize cytoplasmic antigens. Furthermore, they raise the possibility that some nonnuclear proteins may be transported to the nucleus by interacting with proteins harboring nuclear location signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.