ArticleThe Journal of cell biology1986
Purification and characterization of a 190-kD microtubule-associated protein from bovine adrenal cortex.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 70 citations in OpenAlex.
- Mechanical properties of doubly stabilized microtubule filaments.Biophysical journal · 2013Article
- Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.The Journal of cell biology · 1995Article
- In situ localization with digoxigenin-labelled probes of tau-related mRNAs in the rat pancreas.The Histochemical journal · 1995Article
- Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics.The Journal of cell biology · 1995Article
- Pancreatic tau related maps: biochemical and immunofluorescence analysis in a tumoral cell line.Molecular and cellular biochemistry · 1995Article
- Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells.The Journal of cell biology · 1994Article
- Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells.The Journal of cell biology · 1993Article
- Analysis of the primary sequence and microtubule-binding region of the Drosophila 205K MAP.The Journal of cell biology · 1990Article
- Article
- Calcium-mediated modulation of microtubule assembly in human breast epithelial cells.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Article
- High-Mr microtubule-associated proteins: properties and functions.The Biochemical journal · 1989Review
- Immunohistochemical localization of microtubule-associated proteins in the nervous system of the small intestine of guinea pig.Cell and tissue research · 1989Article
- Tau proteins: the molecular structure and mode of binding on microtubules.The Journal of cell biology · 1988Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A heat-stable microtubule-associated protein (MAP) with molecular weight of 190,000, termed 190-kD MAP, was purified from bovine adrenal cortex. This MAP showed the same level of ability to promote tubulin polymerization as did MAP2 and tau from mammalian brains. Relatively high amounts of 190-kD MAP could bind to microtubules reconstituted in the presence of taxol. At maximum 1 mol of 190-kD MAP could bind to 2.3 mol of tubulin. 190-kD MAP was phosphorylated by a cAMP-dependent protein kinase prepared from sea urchin spermatozoa and by protein kinase(s) present in the microtubule protein fraction prepared from mammalian brains. The maximal numbers of incorporated phosphate were approximately 0.2 and approximately 0.4 mol per mole of 190-kD MAP, respectively. These values were lower than that of MAP2, which could be heavily phosphorylated by the endogenous protein kinase(s) up to 5 mol per mole of MAP2 under the same assay condition. 190-kD MAP had no effects on the low-shear viscosity of actin and did not induce an increase in turbidity of the actin solution. It was also revealed that 190-kD MAP does not cosediment with actin filaments. These data clearly show that, distinct from MAP2 and tau, this MAP does not interact with actin. Electron microscopic observation of the rotary-shadowed images of 190-kD MAP showed the molecular shape to be a long, thin, flexible rod with a contour length of approximately 100 nm. Quick-freeze, deep-etch replicas of the microtubules reconstituted from 190-kD MAP and brain tubulin revealed many cross-bridges connecting microtubules with each other.
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