Evidence map›Paper›PMID 37822083›Full record

ArticlePlant biotechnology journal2024

Genome editing in rice using CRISPR/Cas12i3.

Ping Lv, Fei Su, Fangyuan Chen, Chunxue Yan, Dandan Xia, Hui Sun, Shanshan Li, Zhiqiang Duan, Changle Ma, Hui Zhang and 4 more

Open access · goldAbstract read
In one paragraph

Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Base editing in rice using nuclease-deactivated CRISPR/Cas-SF01.Journal of integrative plant biology · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Advances and Applications of Plant Base Editing Technologies.International journal of molecular sciences · 2025
    Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025
    Review
  17. Review
  18. Genome Editing in Mouse Embryo Using the CRISPR/Cas12i3 System.International journal of molecular sciences · 2025
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Ping Lv *Bellagen Biotechnology Co. Ltd, Ji'nan, China.
Fei Su *Bellagen Biotechnology Co. Ltd, Ji'nan, China.
Fangyuan ChenBellagen Biotechnology Co. Ltd, Ji'nan, China.
Chunxue YanBellagen Biotechnology Co. Ltd, Ji'nan, China.
Dandan XiaBellagen Biotechnology Co. Ltd, Ji'nan, China.
Hui SunBellagen Biotechnology Co. Ltd, Ji'nan, China.
Shanshan LiBellagen Biotechnology Co. Ltd, Ji'nan, China.
Zhiqiang DuanBellagen Biotechnology Co. Ltd, Ji'nan, China.
Changle MaSchool of Life Sciences, Shandong Normal University, Ji'nan, China.ORCID 0000-0003-3360-2799
Hui ZhangCollege of Life Science, Shanghai Normal University, Shanghai, China.ORCID 0000-0002-4929-8317
Mugui WangNational Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China.
Xiaomu NiuBellagen Biotechnology Co. Ltd, Ji'nan, China.
Jian-Kang ZhuCenter for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing, China.
Jinshan ZhangBellagen Biotechnology Co. Ltd, Ji'nan, China.ORCID 0000-0001-7360-6837
Shandong Normal University · CNKai Biotech (South Korea) · KRChinese Academy of Agricultural Sciences · CNShanghai Normal University · CNSouthern University of Science and Technology · CN

Funding

Key Research and Development Program of Shandong Province 2021LZGC012Key Research and Development Program of Shandong Province 2022LZGC001
6 · The paper itself

Abstract

The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.

Indexed as

Gene EditingOryzaCRISPR-Cas SystemsEndonucleasesPlantsEndonucleasesBase editorsCRISPR/Cas12i3DNA structural variationsMultiplex DR-spacer Array

Identifiers

PMID37822083
PMCPMC10826996
OpenAlexW4387562854

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.