ArticleClinical epigenetics2023
Accelerated epigenetic age, inflammation, and gene expression in lung: comparisons of smokers and vapers with non-smokers.
Article in Clinical epigenetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Advances in Molecular Research of Tracheobronchial Tree Aging: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Lung lipids associated with smoking and ECIG use in a cross-sectional study and clinical trial.Respiratory research · 2025Trial
- Electronic cigarettes, smoking and lung cancer risk: some evidence about the continuum of harm.Nature reviews. Clinical oncology · 2026Article
- Biological aging acceleration associated with electronic cigarette use in the United States.Environmental epidemiology (Philadelphia, Pa.) · 2026Article
- Substance use and epigenetic age acceleration: Associations with alcohol, tobacco, illicit drugs, and SUD severity.Drug and alcohol dependence · 2026Article
- Exposure to electronic cigarette aerosols triggers alterations in genomic DNA methylation that impacts cancer pathways in mice.Inhalation toxicology · 2026Article
- Vigorous Physical Activity Mitigates Susceptibility to Obesity Associated with Risk Genotypes ofEpigenomes · 2026Article
- Interferon-related inflammaging links epigenetic age acceleration to multimorbidity.Cell genomics · 2026Article
- Lung microbiome predictors of epigenetic aging and potential associations with smoking and electronic cigarette use.Clinical epigenetics · 2026Article
- Article
- How epigenetic clocks tick: Unpacking the black box by deciphering biological pathways and transcriptomic signatures of accelerated aging.Research square · 2026Article
- Multidimensional exposure architecture shapes vaping-associated transcriptomic dysregulation in oral epithelium.Frontiers in oncology · 2026Article
- Blood transcriptomic associations of epigenetic age in adolescents.Epigenetics · 2025Article
- Different dimensions of smoking behavior and their associations with accelerated composite biomarkers-based biological aging in Chinese older adults.BMC geriatrics · 2025Article
- Epigenetic Alterations Induced by Smoking and Their Intersection with Artificial Intelligence: A Narrative Review.International journal of environmental research and public health · 2025Review
- Differential contributions of cardiovascular health-related lifestyle factors to epigenetic ageing: implications for healthy longevity.BMC medicine · 2025Article
- Associations of volatile organic compounds with accelerated epigenetic aging in the lungs of smokers and electronic cigarette users.The Science of the total environment · 2025Article
- Epigenetic clocks as mediators of health behaviors and mortality in middle-aged and older adults.The journal of nutrition, health & aging · 2025Article
- The molecular impact of cigarette smoking resembles aging across tissues.Genome medicine · 2025Article
- Clinical Impact of Vaping.Toxics · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCigarette smoking and aging are the main risk factors for pulmonary diseases, including cancer. Epigenetic aging may explain the relationship between smoking, electronic cigarette vaping, and pulmonary health. No study has examined smoking and vaping-related epigenetic aging in relation to lung biomarkers.
methodsLung epigenetic aging measured by DNA methylation (mAge) and its acceleration (mAA) was assessed in young (age 21-30) electronic cigarette vapers (EC, n = 14, including 3 never-smoking EC), smokers (SM, n = 16), and non-EC/non-SM (NS, n = 39). We investigated relationships of mAge estimates with chronological age (Horvath-mAge), lifespan/mortality (Grim-mAge), telomere length (TL-mAge), smoking/EC history, urinary biomarkers, lung cytokines, and transcriptome.
resultsCompared to NS, EC and SM had significantly older Grim-mAge, shorter TL-mAge, significantly accelerated Grim-mAge and decelerated TL-mAge. Among SM, Grim-mAA was associated with nicotine intake and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL). For EC, Horvath-mAA was significantly correlated with puffs per day. Overall, cytokines (IL-1β, IL-6, and IL-8) and 759 transcripts (651 unique genes) were significantly associated with Grim-mAA. Grim-mAA-associated genes were highly enriched in immune-related pathways and genes that play a role in the morphology and structures of cells/tissues.
conclusionsFaster lung mAge for SM is consistent with prior studies of blood. Faster lung mAge for EC compared to NS indicates possible adverse pulmonary effects of EC on biological aging. Our findings support further research, particularly on epigenetic markers, on effects of smoking and vaping on pulmonary health. Given that most EC are former smokers, further study is needed to understand unique effects of electronic cigarettes on biological aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.