Evidence map›Paper›PMID 37821730›Full record

ArticleTropical animal health and production2023

Comparative study of HA and HNB staining RT-LAMP assays for peste des petits ruminants virus detection in West African Dwarf goats.

Ismaila Muritala, Martha N Bemji, Michael O Ozoje, Olusola L Ajayi, Eniope B Oluwayinka, Adekayode O Sonibare, Ikechukwu J James, Eveline M Ibeagha-Awemu

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Article in Tropical animal health and production, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Ismaila MuritalaDepartment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Martha N BemjiDepartment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. bemjimn@funaab.edu.ng.ORCID http://orcid.org/0000-0001-9740-9854
Michael O OzojeDepartment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Olusola L AjayiDepartment of Pathology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Eniope B OluwayinkaDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Adekayode O SonibareDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Ikechukwu J JamesDepartment of Animal Physiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Eveline M Ibeagha-AwemuSherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec, Canada.
Federal University of Agriculture · NGAgriculture and Agri-Food Canada · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peste des petits ruminants (PPR) cause severe economic losses to many countries of the world where the disease is endemic. It has been targeted for global eradication by 2030 following the successful eradication of rinderpest in 2011. The proposed eradication program would benefit from efficient and relatively reliable diagnostic tools for early PPR virus (PPRV) detection. A total of 33 eight to 12 months old West African Dwarf (WAD) goats were used. Nineteen goats infected by commingling with two PPR virus-positive animals formed the infected group (PPRV-infected goats) while 14 non-infected goats formed the control group (CTG). The suitability of hydroxyl naphthol blue (HNB) staining of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and haemagglutination (HA) assays was compared for their sensitivity to detect the PPRV in PPRV-infected goats and non-infected CTG. PPR disease severity in WAD goats at different days post infection (dpi) was evaluated by clinical scoring and haemagglutination titre (HAT). HNB staining RT-LAMP reaction and HA showed sensitivities of 100% and 73.68%, respectively, for PPRV detection. Expression of PPR clinical signs began from 3 dpi, attained peak at 5 dpi, thereafter showed irregular patterns till 24 dpi. Evaluation of HAT in PPRV-infected goats at 12 dpi ranged from 2 to 64 haemagglutination units (HAU), while CTG goats had 0 HAU. In conclusion, HA could be a good tool for rapid diagnosis of PPRV in a developing country setting. However, HNB staining RT-LAMP assay demonstrated high sensitivity for accurate diagnoses of PPRV and as an important diagnostic tool when precise phenotyping is desired.

Indexed as

Goat DiseasesPeste-des-Petits-RuminantsPeste-des-petits-ruminants virusAnimalsGoatsHemagglutinationMolecular Diagnostic TechniquesNaphtholsNucleic Acid Amplification TechniquesStaining and LabelingNaphtholsClinical scoringHaemagglutination assayHNB staining RT-LAMPPeste des petits ruminantsWest African Dwarf goats

Identifiers

PMID37821730
OpenAlexW4387516589

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.