Evidence map›Paper›PMID 37821714›Full record

ArticleMolecular imaging and biology2024

Imaging Reactive Oxygen Radicals in Excised Mouse Lung Trapped by Reaction with Hydroxylamine Probes Using 1 GHz Rapid Scan Electron Paramagnetic Resonance.

Hanan B Elajaili, Lukas B Woodcock, Tanden A Hovey, George A Rinard, Samuel DeGraw, Autumn Canny, Nathan M Dee, Joseph P Y Kao, Eva S Nozik, Sandra S Eaton and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular imaging and biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Hanan B Elajaili *Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, University of Colorado Anschutz Medical Campus, 12700 E. 19th Ave., B131, Aurora, CO, 80045, USA.
Lukas B Woodcock *Department of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
Tanden A HoveyDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
George A RinardDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
Samuel DeGrawDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
Autumn CannyDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
Nathan M DeeCardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, University of Colorado Anschutz Medical Campus, 12700 E. 19th Ave., B131, Aurora, CO, 80045, USA.
Joseph P Y KaoCenter for Biomedical Engineering and Technology and Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
Eva S NozikCardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, University of Colorado Anschutz Medical Campus, 12700 E. 19th Ave., B131, Aurora, CO, 80045, USA.
Sandra S EatonDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA.
Gareth R EatonDepartment of Chemistry and Biochemistry, University of Denver, Denver, CO, 80210, USA. Gareth.eaton@du.edu.ORCID 0000-0001-7429-8469
University of Denver · USUniversity of Colorado Anschutz Medical Campus · USUniversity of Maryland, Baltimore · US

Funding

SOD3 regulation of redox sensitive signaling in pulmonary vascular diseasesR35HL139726 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI NOZIK, EVA S. · 2018 to 2024
$4.1M
Preclinical Electron Paramagnetic Resonance Tumor ImagerR01CA262159 · NCI · UNIVERSITY OF DENVER (COLORADO SEMINARY) · PI EATON, GARETH R · 2021 to 2025
$2.6M
Developing EPR tools for preclinical interrogation of redox regulation mechanisms contributing to acute lung injuryR33HL157907 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI EATON, SANDRA S., NOZIK, EVA S. · 2021 to 2022
$787k
NCI NIH HHS R01 CA262159NHLBI NIH HHS R33 HL157907NHLBI NIH HHS R35 HL139726NIH HHS R01 CA1262159NIH HHS R33 HL157907
6 · The paper itself

Abstract

purposeOxidative stress is proposed to be critical in acute lung disease, but methods to monitor radicals in lungs are lacking. Our goal is to develop low-frequency electron paramagnetic resonance (EPR) methods to monitor radicals that contribute to the disease. PROCEDURES: Free radicals generated in a lipopolysaccharide-induced mouse model of acute respiratory distress syndrome reacted with cyclic hydroxylamines CPH (1-hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine hydrochloride) and DCP-AM-H (4-acetoxymethoxycarbonyl-1-hydroxy-2,2,5,5-tetramethylpyrrolidine-3-carboxylic acid), which were converted into the corresponding nitroxide radicals, CP• and DCP•. The EPR signals of the nitroxide radicals in excised lungs were imaged with a 1 GHz EPR spectrometer/imager that employs rapid scan technology.

resultsThe small numbers of nitroxides formed by reaction of the hydroxylamine with superoxide result in low signal-to-noise in the spectra and images. However, since the spectral properties of the nitroxides are known, we can use prior knowledge of the line shape and hyperfine splitting to fit the noisy data, yielding well-defined spectra and images. Two-dimensional spectral-spatial images are shown for lung samples containing (4.5 ± 0.5) ×10

conclusionThe nitroxide radicals in excised mouse lungs formed by reaction with hydroxylamine probes CPH and DCP-AM-H can be imaged at 1 GHz.

Indexed as

HydroxylamineLungReactive Oxygen SpeciesAnimalsElectron Spin Resonance SpectroscopyMaleMiceMice, Inbred C57BLNitrogen OxidesHydroxylamineNitrogen OxidesReactive Oxygen SpeciesAcute respiratory distress syndromeNitroxideRapid scan electron paramagnetic resonanceSpin probeSuperoxide

Identifiers

PMID37821714
PMCPMC11006821
OpenAlexW4387522800

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.