ArticleThe Plant cell2024
XYLEM CYSTEINE PEPTIDASE 1 and its inhibitor CYSTATIN 6 regulate pattern-triggered immunity by modulating the stability of the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Identifying anthracnose resistance genes involving in ROS and JA accumulation.Plant signaling & behavior · 2026Article
- CitPH4 Confers Resistance to Citrus Canker by Activating Papain-Like Cysteine Protease.Plant biotechnology journal · 2026Article
- SsPit2A/B Effectors fromPlants (Basel, Switzerland) · 2026Article
- Phytomelatonin receptor PMTR1 is crucial for melatonin-mediated plant broad-spectrum disease resistance.Plant cell reports · 2026Article
- Effector-Mediated Spatial Reprogramming of Glycolate Oxidase Subverts Peroxisomal and Membrane-Associated ROS Defences.Plant biotechnology journal · 2026Article
- The apoplastic protein OsCPIP8 confers dual protection by inhibiting pathogenic protease and activating rice immunity.Nature communications · 2025Article
- Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Salicylic acid-mediated competitive regulation of xylem vessel formation and defense responses in Arabidopsis thaliana.Plant & cell physiology · 2025Article
- Genome-wide molecular characterization and expression profiling of the cysteine protease gene family in maize.BMC genomics · 2025Article
- Plasma membrane CYBDOM proteins catalyse apoplastic AsA regeneration and interact with RbohD to activate autophagy and drought tolerance in plants.Nature plants · 2025Article
- Proteolysis in plant immunity.The Plant cell · 2024Review
- Keeping one's bursts under control: A protease/inhibitor switch regulates reactive oxygen species production during immunity.The Plant cell · 2024Article
- Mitogen-activated protein kinase phosphatase 1 controls broad spectrum disease resistance inFrontiers in plant science · 2024Article
- Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected 'Kinnow' mandarin plants.Frontiers in plant science · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Plants produce a burst of reactive oxygen species (ROS) after pathogen infection to successfully activate immune responses. During pattern-triggered immunity (PTI), ROS are primarily generated by the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD). RBOHD is degraded in the resting state to avoid inappropriate ROS production; however, the enzyme mediating RBOHD degradation and how to prevent RBOHD degradation after pathogen infection is unclear. In this study, we identified an Arabidopsis (Arabidopsis thaliana) vacuole-localized papain-like cysteine protease, XYLEM CYSTEINE PEPTIDASE 1 (XCP1), and its inhibitor CYSTATIN 6 (CYS6). Pathogen-associated molecular pattern-induced ROS burst and resistance were enhanced in the xcp1 mutant but were compromised in the cys6 mutant, indicating that XCP1 and CYS6 oppositely regulate PTI responses. Genetic and biochemical analyses revealed that CYS6 interacts with XCP1 and depends on XCP1 to enhance PTI. Further experiments showed that XCP1 interacts with RBOHD and accelerates RBOHD degradation in a vacuole-mediated manner. CYS6 inhibited the protease activity of XCP1 toward RBOHD, which is critical for RBOHD accumulation upon pathogen infection. As CYS6, XCP1, and RBOHD are conserved in all plant species tested, our findings suggest the existence of a conserved strategy to precisely regulate ROS production under different conditions by modulating the stability of RBOHD.
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