Evidence map›Paper›PMID 37819514›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Fluorescence-based Evaluation of Cyclic di-GMP Levels in Pseudomonas aeruginosa.

Silvia Santoro, Giovanni Bertoni, Silvia Ferrara

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Silvia SantoroDepartment of Biosciences, Università degli Studi di Milano, Milan, Italy.
Giovanni BertoniDepartment of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy.
Silvia FerraraDepartment of Biosciences, Università degli Studi di Milano, Milan, Milano, Italy. silvia.ferrara@unimi.it.
University of Milan · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability of Pseudomonas aeruginosa to establish chronic infections is associated with an effective switch from a motile to a sessile lifestyle. This proficiency is controlled by intracellular levels of the second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). Targeting the c-di-GMP network could be a strategy to interfere with P. aeruginosa pathogenicity. Therefore, the development of tools to profile c-di-GMP intracellular levels is crucial. Here, we describe a protocol for the in vivo measurement of c-di-GMP levels in P. aeruginosa.

Indexed as

Escherichia coli ProteinsPseudomonas aeruginosaBacterial ProteinsBiofilmsCyclic GMPFluorescenceGene Expression Regulation, BacterialSecond Messenger SystemsBacterial Proteinsbis(3',5')-cyclic diguanylic acidCyclic GMPEscherichia coli ProteinsBiosensorc-di-GMPDiguanylate cyclaseFluorescencePhosphodiesterasePseudomonas aeruginosaReporter system

Identifiers

PMID37819514
OpenAlexW4387539706

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.