Evidence map›Paper›PMID 37817909›Full record

ArticleBio-protocol2023

GutMap: A New Interface for Analysing Regional Motility Patterns in ex vivo Mouse Gastrointestinal Preparations.

Tanya Abo-Shaban, Chalystha Y Q Lee, Suzanne Hosie, Gayathri K Balasuriya, Mitra Mohsenipour, Leigh A Johnston, Elisa L Hill-Yardin

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Tanya Abo-ShabanSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
Chalystha Y Q LeeSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
Suzanne HosieSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
Gayathri K BalasuriyaSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
Mitra MohsenipourSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
Leigh A JohnstonDepartment of Biomedical Engineering and Melbourne Brain Centre Imaging Unit, The University of Melbourne, Melbourne, VIC, Australia.
Elisa L Hill-YardinSchool of Health and Biomedical Sciences, STEM College, RMIT University, Bundoora, VIC, Australia.
RMIT University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Different regions of the gastrointestinal tract have specific functions and thus distinct motility patterns. Motility is primarily regulated by the enteric nervous system (ENS), an intrinsic network of neurons located within the gut wall. Under physiological conditions, the ENS is influenced by the central nervous system (CNS). However, by using ex vivo organ bath experiments, ENS regulation of gut motility can also be studied in the absence of CNS influences. The current technique enables the characterisation of small intestinal, caecal, and colonic motility patterns using an ex vivo organ bath and video imaging protocol. This approach is combined with the novel edge detection script GutMap, available in MATLAB, that functions across Windows and Mac platforms. Dissected intestinal segments are cannulated in an organ bath containing physiological saline with a camera mounted overhead. Video recordings of gut contractions are then converted to spatiotemporal heatmaps and analysed using the GutMap software interface. Using data analysed from the heatmaps, parameters of contractile patterns (including contraction propagation frequency and velocity as well as gut diameter) at baseline and in the presence of drugs/treatments/genetic mutations can be compared. Here, we studied motility patterns of female mice at baseline and in the presence of a nitric oxide synthase inhibitor (

Indexed as

Edge detectionEnteric nervous systemEx vivo gut motilityGastrointestinal motilitySoftwareSpatiotemporal heatmap

Identifiers

PMID37817909
PMCPMC10560633
OpenAlexW4387369941

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.