ArticleRapid communications in mass spectrometry : RCM2023
Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization.
Article in Rapid communications in mass spectrometry : RCM, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- MALDI Mass Spectrometry Imaging in Alzheimer's Disease Lipidomics: Matrix Selection, Spatial Lipid Pathology and Emerging Analytical Strategies.International journal of molecular sciences · 2026Review
- Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- One section, two worlds: single-cell integration of MALDI-MSI and spatial transcriptomics on the same single tissue section.Scientific reports · 2025Article
- Demonstrating voxel-by-voxel (V × V) single-point calibration in liver tissue by IR-MALDESI quantitative MSI.Analytical and bioanalytical chemistry · 2025Article
- Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction.Analytical and bioanalytical chemistry · 2025Article
- Feasibility of IR-MALDESI Mass Spectrometry Imaging of PFAS.Journal of mass spectrometry : JMS · 2025Article
- Laser capture microdissection.Nature reviews. Methods primers · 2025Article
- Quantitative sampling by IR-MALDESI is not susceptible to tissue heterogeneity in a multi-organ model.Analytical and bioanalytical chemistry · 2025Article
- Absolute Quantification of Glutathione Using Top-Hat Optics for IR-MALDESI Mass Spectrometry Imaging.Journal of mass spectrometry : JMS · 2024Article
- Improved detection in untargeted lipidomics through silver-doped infrared matrix-assisted laser desorption electrospray ionization.Rapid communications in mass spectrometry : RCM · 2024Article
- Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI.Journal of the American Society for Mass Spectrometry · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
rationaleInfrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) utilizes a 2970 nm mid-IR laser to desorb samples with depth resolutions (Z) on the order of micrometers. Conventionally, 5-20 μm thick tissue sections are used to characterize different applications of the IR-MALDESI source, but an optimal thickness has not been systematically investigated.
methodsMouse liver was sectioned to various thicknesses and analyzed using IR-MALDESI mass spectrometry imaging (MSI). Height profiles of tissue sections of various cryosectioned thicknesses were acquired to affirm tissue thickness. Tissue sections of each thickness were measured using a Keyence microscope. Paraffin wax was cryosectioned, mounted on microscope slides, and measured using a chromatic confocal sensor system to determine the cryostat sectioning accuracy.
resultsAnalyzing sectioned tissues at higher thickness (>10 μm) leads to lower ion abundance, a decrease in signal over long analysis times, and more frequent instrument cleaning. Additionally, increasing tissue thickness above the optimum (7 μm) does not result in a significant increase in lipid annotations.
conclusionsThis work defines an optimal sample thickness for IR-MALDESI-MSI and demonstrates the utility of optimizing tissue thickness for MSI platforms of comparable Z resolution.
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