Evidence map›Paper›PMID 37817254›Full record

ReviewJournal of nanobiotechnology2023

A potential paradigm in CRISPR/Cas systems delivery: at the crossroad of microalgal gene editing and algal-mediated nanoparticles.

Shuying Feng, Xin Xie, Junjie Liu, Aifang Li, Qianqian Wang, Dandan Guo, Shuxuan Li, Yalan Li, Zilong Wang, Tao Guo and 3 more

Open access · goldAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of nanobiotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Shuying Feng *Medical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China. fsy@hactcm.edu.cn.
Xin Xie *Medical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Junjie Liu *Medical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Aifang LiMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Qianqian WangMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Dandan GuoMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Shuxuan LiMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Yalan LiMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Zilong WangMedical College, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China.
Tao GuoDepartment of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China. gt010010@163.com.
Jin ZhouInstitute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong, China. zhou.jin@sz.tsinghua.edu.cn.
Doris Ying Ying TangDepartment of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500, Semenyih, Malaysia.
Pau Loke ShowDepartment of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates. PauLoke.Show@ku.ac.ae.
First Affiliated Hospital of Henan University · CNKhalifa University of Science and Technology · AEUniversity of Nottingham Malaysia Campus · MY

Funding

Basic Research Project of the Key Research Program of Colleges and Universities in Henan Province 23ZX005National Natural Science Foundation of China No. U1804112Natural Science Foundation of Henan Province 232300421164
6 · The paper itself

Abstract

Microalgae as the photosynthetic organisms offer enormous promise in a variety of industries, such as the generation of high-value byproducts, biofuels, pharmaceuticals, environmental remediation, and others. With the rapid advancement of gene editing technology, CRISPR/Cas system has evolved into an effective tool that revolutionised the genetic engineering of microalgae due to its robustness, high target specificity, and programmability. However, due to the lack of robust delivery system, the efficacy of gene editing is significantly impaired, limiting its application in microalgae. Nanomaterials have become a potential delivery platform for CRISPR/Cas systems due to their advantages of precise targeting, high stability, safety, and improved immune system. Notably, algal-mediated nanoparticles (AMNPs), especially the microalgae-derived nanoparticles, are appealing as a sustainable delivery platform because of their biocompatibility and low toxicity in a homologous relationship. In addition, living microalgae demonstrated effective and regulated distribution into specified areas as the biohybrid microrobots. This review extensively summarised the uses of CRISPR/Cas systems in microalgae and the recent developments of nanoparticle-based CRISPR/Cas delivery systems. A systematic description of the properties and uses of AMNPs, microalgae-derived nanoparticles, and microalgae microrobots has also been discussed. Finally, this review highlights the challenges and future research directions for the development of gene-edited microalgae.

Indexed as

MicroalgaeNanoparticlesCRISPR-Cas SystemsGene EditingGenetic EngineeringAlgal-mediated nanoparticlesCRISPR/Cas systemDelivery systemGene editingMicroalgae application

Identifiers

PMID37817254
PMCPMC10563294
OpenAlexW4387501516

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.