ArticleMicrobial cell factories2023
The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum.
Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 13 citations in OpenAlex.
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- Physio-Morphological Assessment and Multi-Omics Analysis ofJournal of fungi (Basel, Switzerland) · 2026Article
- Genome-wide association studies revealed association between LePC4 gene and crude protein content in shiitake mushroom, Lentinula edodes.World journal of microbiology & biotechnology · 2026Article
- Unraveling the Signaling Networks: How Exogenous Substances Mitigate Heat Stress in Edible Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- The Metabolic Regulatory Mechanisms of Umami Amino Acids inFoods (Basel, Switzerland) · 2026Article
- The Aromatic Amino Acid Biosynthesis GeneMicroorganisms · 2025Article
- Article
- Developmental and reproductive toxicity assessment of sporoderm-removedFrontiers in cell and developmental biology · 2025Article
- Research progress on the function and regulatory pathways of amino acid permeases in fungi.World journal of microbiology & biotechnology · 2024Review
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- Genetic regulation of L-tryptophan metabolism in Psilocybe mexicana supports psilocybin biosynthesis.Fungal biology and biotechnology · 2024Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
backgroundEdible mushrooms are delicious in flavour and rich in high-quality protein and amino acids required by humans. A transcription factor, general control nonderepressible 4 (GCN4), can regulate the expression of genes involved in amino acid metabolism in yeast and mammals. A previous study revealed that GCN4 plays a pivotal role in nitrogen utilization and growth in Ganoderma lucidum. However, its regulation is nearly unknown in mushrooms.
resultsIn this study, we found that the amino acid contents reached 120.51 mg per gram of mycelia in the WT strain under 60 mM asparagine (Asn) conditions, but decreased by 62.96% under 3 mM Asn conditions. Second, silencing of gcn4 resulted in a 54.2% decrease in amino acid contents under 60 mM Asn, especially for the essential and monosodium glutamate-like flavour amino acids. However, these effects were more pronounced under 3 mM Asn. Third, silencing of gcn4 markedly inhibited the expression of amino acid biosynthesis and transport genes. In addition, GCN4 enhanced the tricarboxylic acid cycle (TCA) and glycolytic pathway and inhibited the activity of target of rapamycin complex 1 (TORC1), thus being beneficial for maintaining amino acid homeostasis.
conclusionThis study confirmed that GCN4 contributes to maintaining the amino acid contents in mushrooms under low concentrations of nitrogen. In conclusion, our study provides a research basis for GCN4 to regulate amino acid synthesis and improve the nutrient contents of edible mushrooms.
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