Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography.

Nicole A Cheung, Mabel Song, Christopher K Sue, Robert T Clubb

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nicole A CheungUCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, Los Angeles, CA, USA.

Mabel SongDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.

Christopher K SueDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.

Robert T ClubbDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA. rclubb@mbi.ucla.edu.

Funding

Cell Surface Protein Anchoring in Gram-Positive BacteriaR01AI052217 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2002 to 2026

$8.5M

Research Training in Cell and Molecular BiologyT32GM145388 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Jorge Torres · 2022 to 2026

$5.2M

NIAID NIH HHS R01 AI052217NIGMS NIH HHS T32 GM145388

6 · The paper itself

Abstract

Gram-positive bacteria display pili whose protein components (pilins) are covalently crosslinked by pilus-specific sortase enzymes. These cysteine transpeptidase enzymes catalyze a transpeptidation reaction that joins the pilins together via lysine isopeptide bonds. The crosslinking reaction that builds the SpaA pilus in Corynebacterium diphtheriae is mediated by the SrtA sortase (

Indexed as

AminoacyltransferasesFimbriae ProteinsBacterial ProteinsCadmiumCatalysisChromatography, High Pressure LiquidFimbriae, BacterialAminoacyltransferasesBacterial ProteinsCadmiumFimbriae ProteinsHigh-performance liquid chromatographyKineticsPiliSortase

Identifiers

PMID37815714

DOI10.1007/978-1-0716-3491-2_11

PMCPMC12629098

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.