Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris.

Aadil H Bhat, Asis Das, Hung Ton-That

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.8field-weighted citation impact, top 22% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Molecular basis for sortase-catalyzed pilus tip assembly.mBio · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Aadil H BhatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, CA, USA.

Asis DasDepartment of Medicine, Neag Comprehensive Cancer Center, University of Connecticut Health Center, Farmington, CT, USA.

Hung Ton-ThatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, CA, USA. htonthat@dentistry.ucla.edu.

University of California, Los Angeles · USUConn Health · US

Funding

Molecular Assembly on the Cell Surface of ActinomycesR01DE017382 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI Hung Ton-That · 2008 to 2026

$6.7M

NIDCR NIH HHS R01 DE017382

6 · The paper itself

Abstract

The Gram-positive bacterium Actinomyces oris expresses a unique cell wall-anchored fimbria comprised of the fimbrial shaft FimA and the tip fimbrillin CafA, whose gene is not genetically linked to the fimA locus, unlike many other fimbrial gene loci in Gram-positive bacteria. Mutational analyses of individual fimbrillins, FimA and CafA, in A. oris often rely on multi-copy plasmids that may alter the stoichiometry of fimbrillins in vivo, hence fimbrial assembly. Here, we provide a robust method for single-copy gene expression and mutagenesis in A. oris, using CafA as an experimental model. This method can be applied for single-copy gene editing in various bacterial systems.

Indexed as

Fimbriae ProteinsGene EditingActinomycesBacterial ProteinsCell WallFimbriae, BacterialBacterial ProteinsFimbriae ProteinsActinomyces orisCo-integrationHomologous recombinationMutagenesisPilinRoom temperature transformation

Identifiers

PMID37815713

DOI10.1007/978-1-4939-3676-2_9

OpenAlexW4387473013

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.