Evidence map›Paper›PMID 37815706›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy.

Kevin To, Timmie Britton, Hung Ton-That

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Kevin To *Division of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, CA, USA.
Timmie Britton *Molecular Biology Institute, University of California, Los Angeles, CA, USA.
Hung Ton-ThatDivision of Oral & Systemic Health Sciences, School of Dentistry, University of California, Los Angeles, CA, USA. htonthat@dentistry.ucla.edu.
University of California, Los Angeles · USUCLA Health · US

Funding

Virulence determinants of Fusobacterium nucleatumR01DE026758 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI TON-THAT, HUNG · 2017 to 2021
$2.0M
NIDCR NIH HHS R01 DE026758
6 · The paper itself

Abstract

Innately present in tears, saliva and mucosal secretions, lysozyme provides a critical defensive strategy to the host by cleaving the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine residues of peptidoglycan of invading bacteria, leading to bacterial lysis. To counter this class of cell wall hydrolase enzymes, bacteria produce several lysozyme inhibitors, a representative of which, MliC, was identified in Escherichia coli, Pseudomonas aeruginosa, and various bacterial species. The Gram-negative oral anaerobe Fusobacterium nucleatum encodes an uncharacterized lipoprotein homologous to MliC, whose localization is unknown. Here, we provide an experimental procedure to localize this MliC-like lipoprotein by employing immunofluorescence microscopy. In principle, this protocol can be used for any bacterial system to monitor protein localization.

Indexed as

Fusobacterium nucleatumMuramidaseBacteriaCell WallEscherichia coliLipoproteinsLipoproteinsMuramidaseCell wall hydrolaseFusobacterium nucleatumGram-negative bacteriaImmunofluorescenceLysozyme inhibitorMembrane permeabilization

Identifiers

PMID37815706
OpenAlexW4387472517

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.