Evidence map›Paper›PMID 37815704›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall.

Stephen N Hyland, Sreedevi Chinthamani, Sushanta Ratna, Kimberly A Wodzanowski, Liam-Michael D Sandles, Kiyonobu Honma, Catherine Leimkuhler-Grimes, Ashu Sharma

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. ACS infectious diseases · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Stephen N HylandDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
Sreedevi ChinthamaniDepartment of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA.
Sushanta RatnaDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
Kimberly A WodzanowskiDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
Liam-Michael D SandlesDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
Kiyonobu HonmaDepartment of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA.
Catherine Leimkuhler-GrimesDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA. cgrimes@udel.edu.
Ashu SharmaDepartment of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA. sharmaa@buffalo.edu.
University of Delaware · USUniversity at Buffalo, State University of New York · US

Funding

Bacterial sialometabolic activity impacts periodontal immunity and microbiotaR01DE029497 · NIDCR · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI SHARMA, ASHU · 2021 to 2025
$1.8M
NIDCR NIH HHS R01 DE029497
6 · The paper itself

Abstract

The objective of this chapter is to provide a detailed protocol for the peptidoglycan (cell wall) labeling of the periodontal pathogen Tannerella forsythia and the development of a laboratory-safe Escherichia coli strain utilizing the N-acetylmuramic acid recycling enzymes AmgK, N-acetylmuramate/N-acetylglucosamine kinase, and MurU, N-acetylmuramate alpha-1-phosphate uridylyltransferase, from T. forsythia. The procedure involves bioorthogonal labeling of bacterial cells with an azido-modified analog of the amino sugar, N-acetylmuramic acid, through "click chemistry" with a fluorescent dye. The protocol is suitable for the generation of fluorescently labeled peptidoglycan molecules for applications in the study of bacterial and peptidoglycan trafficking in the host cells and cell wall recycling in complex microbiomes.

Indexed as

PeptidoglycanTannerella forsythiaCell WallClick ChemistryEscherichia coliMuramic AcidsMuramic AcidsN-acetylmuramic acidPeptidoglycanBioorthogonalCell wall recyclingClick chemistryMass spectrometryMicroscopyPeptidoglycanTannerella forsythia

Identifiers

PMID37815704
PMCPMC11513787
OpenAlexW4387472395

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.