ArticleNature plants2023
Targeted knockout of a conserved plant mitochondrial gene by genome editing.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Harnessing mitochondrial genome editing for crop improvement: principles and applications.aBIOTECH · 2026Review
- RhoMitoAnnotator and Polypods, Bioinformatics Tools for theInternational journal of molecular sciences · 2026Article
- Breeding mitochondria in crops.Nature plants · 2026Article
- High-frequency biparental inheritance of plant mitochondria upon chilling stress and loss of a genome-degrading nuclease.Nature plants · 2026Article
- Comparative analysis of complete mitogenomes of two ornamental plants Chimonanthus salicifolius and Chimonanthus praecox in the family Calycanthaceae.BMC plant biology · 2026Article
- Advances in Double-Stranded DNA Targeting Technologies.Exploration (Beijing, China) · 2026Review
- Editing mitochondrial and chloroplast genomes : Genome editing technologies for plant organelles provide new opportunities in functional genomics and breeding.EMBO reports · 2026Article
- Nano-enabled plant genetic engineering for stress resilience: current advances and future directions.Frontiers in plant science · 2026Review
- Advancements in genomic crop techniques and considerations for regulation and food safety.Transgenic research · 2025Review
- Advances in Cytoplasmic Male Sterility in Sugar Beet from Mitochondrial Genome Structural Dynamics and Nuclear-Cytoplasmic Coordination.International journal of molecular sciences · 2025Review
- Functional relocation of the maize chloroplast atpB gene to the nucleus restores photosynthetic competence to a gene-edited non-photosynthetic mutant.Plant biotechnology journal · 2025Article
- The evolutionary dynamics of organellar pan-genomes in Arabidopsis thaliana.Genome biology · 2025Article
- Genome modification in plant mitochondria.Plant physiology · 2025Review
- Reverse genetics in the Arabidopsis chloroplast genome identifies rps16 as a transcribed pseudogene.The Plant journal : for cell and molecular biology · 2025Article
- Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato.Plant biotechnology journal · 2025Article
- Targeted translation inhibition of chloroplast and mitochondrial mRNAs by designer pentatricopeptide repeat proteins.Nucleic acids research · 2025Article
- PMGA: A plant mitochondrial genome annotator.Plant communications · 2025Article
- Genome Editing of Plant Mitochondrial and Chloroplast Genomes.Plant & cell physiology · 2024Review
- Conservation of the moss RNA editing factor PPR78 despite the loss of its known cytidine-to-uridine editing sites is explained by a hidden extra target.The Plant cell · 2024Article
- TALE-based organellar genome editing and gene expression in plants.Plant cell reports · 2024Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Fusion proteins derived from transcription activator-like effectors (TALEs) have emerged as genome editing tools for mitochondria. TALE nucleases (TALENs) have been applied to delete chimaeric reading frames and duplicated (redundant) genes but produced complex genomic rearrangements due to the absence of non-homologous end-joining. Here we report the targeted deletion of a conserved mitochondrial gene, nad9, encoding a subunit of respiratory complex I. By generating a large number of TALEN-mediated mitochondrial deletion lines, we isolated, in addition to mutants with rearranged genomes, homochondriomic mutants harbouring clean nad9 deletions. Characterization of the knockout plants revealed impaired complex I biogenesis, male sterility and defects in leaf and flower development. We show that these defects can be restored by expressing a functional Nad9 protein from the nuclear genome, thus creating a synthetic cytoplasmic male sterility system. Our data (1) demonstrate the feasibility of using genome editing to study mitochondrial gene functions by reverse genetics, (2) highlight the role of complex I in plant development and (3) provide proof-of-concept for the construction of synthetic cytoplasmic male sterility systems for hybrid breeding by genome editing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.