Evidence map›Paper›PMID 37813160›Full record

ArticleDevelopmental biology2023

In vivo tracing of the Cytokeratin 14 lineages using self-cleaving guide RNAs and CRISPR/Cas9.

Amita Tiyaboonchai, Leslie Wakefield, Anne Vonada, Catherine L May, Craig Dorrell, David Enicks, Anusha Sairavi, Klaus H Kaestner, Markus Grompe

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 70% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Amita TiyaboonchaiOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Pediatrics, Oregon Health & Science University, Portland, OR, 97239, USA. Electronic address: tiyaboon@ohsu.edu.
Leslie WakefieldOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Pediatrics, Oregon Health & Science University, Portland, OR, 97239, USA.
Anne VonadaOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Molecular and Medical Genetics, Oregon Health & Science University, Portland, OR, 97239, USA.
Catherine L MayInstitute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, 19104, USA; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, 19104, USA.
Craig DorrellOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Pediatrics, Oregon Health & Science University, Portland, OR, 97239, USA.
David EnicksOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Pediatrics, Oregon Health & Science University, Portland, OR, 97239, USA.
Anusha SairaviDepartment of Molecular and Medical Genetics, Oregon Health & Science University, Portland, OR, 97239, USA.
Klaus H KaestnerInstitute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, 19104, USA; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, 19104, USA.
Markus GrompeOregon Stem Cell Center, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Pediatrics, Oregon Health & Science University, Portland, OR, 97239, USA; Department of Molecular and Medical Genetics, Oregon Health & Science University, Portland, OR, 97239, USA.
Oregon Health & Science University · USUniversity of Pennsylvania · US

Funding

VIRAL VECTOR COREP30DK019525 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI DOUGLAS J EPSTEIN · 1986 to 2026
$48.3M
Treatment of Distal Tyrosine Pathway DisordersR01DK048252 · NIDDK · OREGON HEALTH & SCIENCE UNIVERSITY · PI GROMPE, MARKUS · 1994 to 2017
$5.5M
Program in Biomedical SciencesT32GM142619 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI Jeffrey Wallace Tyner · 2021 to 2026
$3.2M
In vivo selection of gene modified hepatocytesR01DK123093 · NIDDK · OREGON HEALTH & SCIENCE UNIVERSITY · PI GROMPE, MARKUS · 2021 to 2023
$1.5M
Enhancing the Gene Ride vector for Liver Gene TherapyF32DK117516 · NIDDK · OREGON HEALTH & SCIENCE UNIVERSITY · PI TIYABOONCHAI, AMITA · 2018 to 2020
$188k
NIDDK NIH HHS F32 DK117516NIDDK NIH HHS P30 DK019525NIDDK NIH HHS R01 DK048252NIDDK NIH HHS R01 DK123093NIGMS NIH HHS T32 GM142619
6 · The paper itself

Abstract

The current gold-standard for genetic lineage tracing in transgenic mice is based on cell-type specific expression of Cre recombinase. As an alternative, we developed a cell-type specific CRISPR/spCas9 system for lineage tracing. This method relies on RNA polymerase II promoter driven self-cleaving guide RNAs (scgRNA) to achieve tissue-specificity. To demonstrate proof-of-principle for this approach a transgenic mouse was generated harbouring a knock-in of a scgRNA into the Cytokeratin 14 (Krt14) locus. Krt14 expression marks the stem cells of squamous epithelium in the skin and oral mucosa. The scgRNA targets a Stop cassette preceding a fluorescent reporter in the Ai9-tdtomato mouse. Ai9-tdtomato reporter mice harbouring this allele along with a spCas9 transgene demonstrated precise marking of the Krt14 lineage. We conclude that RNA polymerase II promoter driven scgRNAs enable the use of CRISPR/spCas9 for genetic lineage tracing.

Indexed as

CRISPR-Cas SystemsRNA Polymerase IIAnimalsIntegrasesKeratin-14MiceMice, TransgenicPromoter Regions, GeneticIntegrasesKeratin-14Krt14 protein, mouseRNA Polymerase II

Identifiers

PMID37813160
PMCPMC11631131
OpenAlexW4387432545

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.