Evidence map›Paper›PMID 37810390›Full record

ArticleFrontiers in plant science2023

CRISPR/Cas9-mediated lipoxygenase gene-editing in yellow pea leads to major changes in fatty acid and flavor profiles.

Pankaj Bhowmik, Wei Yan, Connor Hodgins, Brittany Polley, Tom Warkentin, Michael Nickerson, Dae-Kyun Ro, Frédéric Marsolais, Claire Domoney, Shiva Shariati-Ievari and 1 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
8.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 54 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 2 countries.

Pankaj BhowmikAquatic and Crop Resource Development Centre, National Research Council Canada, Saskatoon, SK, Canada.
Wei YanAquatic and Crop Resource Development Centre, National Research Council Canada, Saskatoon, SK, Canada.
Connor HodginsDepartment of Biological Sciences, University of Calgary, Calgary, AB, Canada.
Brittany PolleyAquatic and Crop Resource Development Centre, National Research Council Canada, Saskatoon, SK, Canada.
Tom WarkentinDepartment of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Michael NickersonDepartment of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Dae-Kyun RoDepartment of Biological Sciences, University of Calgary, Calgary, AB, Canada.
Frédéric MarsolaisLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
Claire DomoneyDepartment of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
Shiva Shariati-IevariDivision of Neurodegenerative Diseases (DND), St Boniface Hospital Research Center, Winnipeg, MB, Canada.
Michel AlianiDivision of Neurodegenerative Diseases (DND), St Boniface Hospital Research Center, Winnipeg, MB, Canada.
National Research Council Canada · CAUniversity of Calgary · CAUniversity of Saskatchewan · CAAgriculture and Agri-Food Canada · CANorwich Research Park · GBSt. Boniface Hospital · CAUniversity of Manitoba · CA

Funding

Biotechnology and Biological Sciences Research Council BBS/E/J/000CA392
6 · The paper itself

Abstract

Introduction: Although pulses are nutritious foods containing high amounts of protein, fiber and phytochemicals, their consumption and use in the food industry have been limited due to the formation of unappealing flavors/aromas described as beany, green, and grassy. Lipoxygenase (LOX) enzymes are prevalent among pulse seeds, and their activity can lead to the formation of specific volatile organic compounds (VOCs) from certain polyunsaturated fatty acids (PUFAs). As a widespread issue in legumes, including soybean, these VOCs have been linked to certain unappealing taste perception of foods containing processed pulse seeds. Methods: To address this problem in pea and as proof of principle to promote the wider use of pulses, a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) construct was designed to create null alleles (knockouts) of Results and discussion: Successful CRISPR/Cas9-mediated LOX gene editing of stable transgenic pea lines (TGP) was confirmed by DNA sequencing of the wild type (WT) and TGP

Indexed as

aromaCRISPR/Cas9fatty acidsflavorgene-editinglipoxygenasepea

Identifiers

PMID37810390
PMCPMC10552856
OpenAlexW4386914078

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.