ArticleHeliyon2023
Genetic modification regulates pathogenicity of a fowl adenovirus 4 strain after cell line adaptation (genetic mutation in FAdV-4 lowered pathogenicity).
Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Neuroimmune responses in the chicken brain during acute fowl adenovirus serotype 4 infection.Frontiers in veterinary science · 2026Article
- Generation of an artificially attenuated fowl adenovirus 4 viral vector using the reverse genetics system based on full-length infectious clone.Veterinary research · 2025Article
- Comparative analysis of changes in immune cell in the chicken spleen across different ages using flow cytometry.BMC veterinary research · 2024Article
- Pathogenicity of Duck Adenovirus Type 3 in Chickens.Animals : an open access journal from MDPI · 2024Article
- Protective immune response induced by Leghorn male hepatoma cell-adapted fowl adenovirus-4.Heliyon · 2024Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fowl adenovirus 4 (FAdV-4) is a major avian virus that induces fatal diseases in chicken such as, hydropericardium and hepatitis. The viral structure consists of hexon, penton, fiber-1, and fiber-2 which are associated with immunopathogenesis. In this study, we investigated the genetic modification of a FAdV-4 strain after continuous passages in a cell line and evaluated the pathogenicity associated with mutations. We used the FadV-4 KNU14061 strain, which was isolated from layers in 2014. The virus went through 80 passages in the Leghorn male hepatoma (LMH) cell line. The full genetic sequence was identified, and we found a frameshift in the fiber-2 amino acid sequence after the initial thirty passages. To examine whether the frameshift in the fiber-2 gene affects the pathogenicity in chicken, we inoculated LMH80 (80 times passaged) and LMH10 (10 times passaged) into 3-day-old chickens and examined the pathogenesis. LMH10 infection via intramuscular route induced fatal pathology, but LMH80 did not. Furthermore, LHM80 pre-treatment protected hosts from the LMH10 challenge. Thus, the genetic modification isolated by serial passage lowered pathogenicity and the resulting virus acted as an attenuated vaccine that can be a FAdV-4 vaccine strain candidate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.