Evidence map›Paper›PMID 37808676›Full record

ArticlebioRxiv : the preprint server for biology2023

Structural basis of Cfr-mediated antimicrobial resistance and mechanisms for its evasion.

Elena V Aleksandrova, Kelvin J Y Wu, Ben I C Tresco, Egor A Syroegin, Erin E Killeavy, Samson M Balasanyants, Maxim S Svetlov, Steven T Gregory, Gemma C Atkinson, Andrew G Myers and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Elena V AleksandrovaDepartment of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Kelvin J Y WuDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Ben I C TrescoDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Egor A SyroeginDepartment of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Erin E KilleavyDepartment of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA.
Samson M BalasanyantsDepartment of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Maxim S SvetlovDepartment of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Steven T GregoryDepartment of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA.
Gemma C AtkinsonDepartment of Experimental Medicine, University of Lund, Lund, Sweden.
Andrew G MyersDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Yury S PolikanovDepartment of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI Nozomi Ando, J Christopher Fromme · 2018 to 2026
$34.2M
Structural Robustness of Ribosome Functional CentersR01GM094157 · NIGMS · BROWN UNIVERSITY · PI GREGORY, STEVEN, JOGL, GERWALD · 2010 to 2024
$4.4M
Discovery through chemical synthesis of antibiotics effective against modern bacterial pathogensR01AI168228 · NIAID · HARVARD UNIVERSITY · PI ANDREW G MYERS · 2022 to 2026
$3.8M
Pixel Array Detector for Macromolecular CrystallographyS10OD021527 · OD · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2016 to 2016
$2.0M
Molecular mechanisms of action of ribosome-targeting antibiotics.R01GM132302 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI POLIKANOV, YURY · 2019 to 2022
$1.3M
Structural basis of Cfr-mediated resistance to antibiotics targeting the bacterial ribosomeR21AI163466 · NIAID · UNIVERSITY OF ILLINOIS AT CHICAGO · PI POLIKANOV, YURY · 2021 to 2022
$426k
NIAID NIH HHS R01 AI168228NIAID NIH HHS R21 AI163466NIGMS NIH HHS P30 GM124165NIGMS NIH HHS R01 GM094157NIGMS NIH HHS R01 GM132302NIH HHS S10 OD021527
6 · The paper itself

Abstract

The ribosome is an essential drug target as many classes of clinically important antibiotics bind and inhibit its functional centers. The catalytic peptidyl transferase center (PTC) is targeted by the broadest array of inhibitors belonging to several chemical classes. One of the most abundant and clinically prevalent mechanisms of resistance to PTC-acting drugs is C8-methylation of the universally conserved adenine residue 2503 (A2503) of the 23S rRNA by the methyltransferase Cfr. Despite its clinical significance, a sufficient understanding of the molecular mechanisms underlying Cfr-mediated resistance is currently lacking. In this work, we developed a method to express a functionally-active Cfr-methyltransferase in the thermophilic bacterium

Indexed as

23S rRNA70S ribosomeA2503antibioticCFRiboxamycininhibition of translationmethylationmethyltransferasemultidrugnascent peptide exit tunnelpeptidyl transferase centerresistancetylosinX-ray structure

Identifiers

PMID37808676
PMCPMC10557674

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.