Evidence map›Paper›PMID 37808667›Full record

ArticlebioRxiv : the preprint server for biology2023

Tenofovir Activation is Diminished in the Brain and Liver of Creatine Kinase Brain-Type Knockout Mice.

Colten D Eberhard, Eric P Mosher, Namandjé N Bumpus, Benjamin C Orsburn

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Colten D EberhardDepartment of Pharmacology and Molecular Sciences Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Eric P MosherDepartment of Pharmacology and Molecular Sciences Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Namandjé N BumpusDepartment of Pharmacology and Molecular Sciences Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Benjamin C OrsburnDepartment of Pharmacology and Molecular Sciences Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Johns Hopkins University · US

Funding

Pharmacology and Molecular Sciences Training Program: Enhancing Inclusivity Through Universal Design for Learning in Graduate CoursesT32GM135083 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Caren L. Freel Meyers · 2020 to 2026
$3.3M
Drug Phosphorylation and AgingR01AG064908 · NIA · JOHNS HOPKINS UNIVERSITY · PI ORSBURN, BENJAMIN CARL · 2020 to 2023
$2.4M
NIA NIH HHS R01 AG064908NIGMS NIH HHS T32 GM135083
6 · The paper itself

Abstract

Tenofovir (TFV) is a nucleotide reverse transcriptase inhibitor prescribed for the treatment and prevention of human immunodeficiency virus infection, and the treatment of chronic hepatitis B virus infection. Here, we demonstrate that creatine kinase brain-type (CKB) can form tenofovir-diphosphate (TFV-DP), the pharmacologically active metabolite, in vitro, and identify nine missense mutations (C74S, R96P, S128R, R132H, R172P, R236Q, C283S, R292Q, and H296R) that diminish this activity. Additional characterization of these mutations reveal that five (R96P, R132H, R236Q, C283S, and R292Q) have ATP dephosphorylation catalytic efficiencies less than 20% of wild-type (WT), and seven (C74S, R96P, R132H, R172P, R236Q, C283S, and H296P) induce thermal instabilities. To determine the extent CKB contributes to TFV activation in vivo, we generated a CKB knockout mouse strain,

Indexed as

creatine kinase brain-typedrug metabolismknockoutmouse modelproteomicstenofovir

Identifiers

PMID37808667
PMCPMC10557616
OpenAlexW4387037359

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.