Evidence map›Paper›PMID 37805554›Full record

ArticleScientific reports2023

A synthetic coolant (WS-23) in disposable electronic cigarettes impairs cytoskeletal function in EpiAirway microtissues exposed at the air liquid interface.

Man Wong, Teresa Martinez, Mona Tran, Cori Zuvia, Alisa Gadkari, Esther E Omaiye, Wentai Luo, Kevin J McWhirter, Jihui Sha, Ahmad Kassem and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Man WongDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Teresa MartinezDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Mona TranDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Cori ZuviaDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Alisa GadkariDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Esther E OmaiyeDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA.
Wentai LuoDepartment of Civil and Environmental Engineering, Portland State University, Portland, OR, 97207, USA.
Kevin J McWhirterDepartment of Civil and Environmental Engineering, Portland State University, Portland, OR, 97207, USA.
Jihui ShaDepartment of Biological Chemistry, David Geffen School of Medicine at University of California, Los Angeles, CA, 90095, USA.
Ahmad KassemDepartment of Biological Chemistry, David Geffen School of Medicine at University of California, Los Angeles, CA, 90095, USA.
James WohlschlegelDepartment of Biological Chemistry, David Geffen School of Medicine at University of California, Los Angeles, CA, 90095, USA.
Prue TalbotDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, CA, 92521, USA. talbot@ucr.edu.
University of California, Riverside · USUniversity of California, Los Angeles · USPortland State University · US

Funding

Identification and toxicity of chemicals that are linked to EVALIR01ES029741 · NIEHS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI TALBOT, PRUDENCE · 2018 to 2022
$3.7M
NIEHS NIH HHS R01 ES029741NIH HHS R01ES029741
6 · The paper itself

Abstract

The design of popular disposable electronic cigarettes (ECs) was analyzed, and the concentrations of WS-23, a synthetic coolant, in EC fluids were determined for 22 devices from 4 different brands. All products contained WS-23 in concentrations that ranged from 1.0 to 40.1 mg/mL (mean = 21.4 ± 9.2 mg/mL). To determine the effects of WS-23 on human bronchial epithelium in isolation of other chemicals, we exposed EpiAirway 3-D microtissues to WS-23 at the air liquid interface (ALI) using a cloud chamber that generated aerosols without heating. Proteomics analysis of exposed tissues revealed that the cytoskeleton was a major target of WS-23. BEAS-2B cells were exposed to WS-23 in submerged culture to validate the main results from proteomics. F-actin, which was visualized with phalloidin, decreased concentration dependently in WS-23 treated BEAS-2B cells, and cells became immotile in concentrations above 1.5 mg/mL. Gap closure, which depends on both cell proliferation and migration, was inhibited by 0.45 mg/mL of WS-23. These data show that WS-23 is being added to popular EC fluids at concentrations that can impair processes dependent on the actin cytoskeleton and disturb homeostasis of the bronchial epithelium. The unregulated use of WS-23 in EC products may harm human health.

Indexed as

Electronic Nicotine Delivery SystemsAerosolsCytoskeletonHumansAerosols

Identifiers

PMID37805554
PMCPMC10560211
OpenAlexW4387426053

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.