Evidence map›Paper›PMID 37805521›Full record

ArticleBMC veterinary research2023

Genetic characterization of Bartonella henselae samples isolated from stray cats by multi-locus sequence typing.

Hüseyin Can, Mervenur Güvendi, Ecem Sürgeç, Ahmet Efe Köseoğlu, Sedef Erkunt Alak, Muhammet Karakavuk, Aytül Gül, Mert Döşkaya, Adnan Yüksel Gürüz, Cemal Ün and 1 more

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Article in BMC veterinary research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Hüseyin CanFaculty of Science, Department of Biology Molecular Biology Section, Ege University, İzmir, Turkey.
Mervenur GüvendiFaculty of Science, Department of Biology Molecular Biology Section, Ege University, İzmir, Turkey.
Ecem SürgeçFaculty of Science, Department of Biology Zoology Section, Ege University, İzmir, Turkey.
Ahmet Efe KöseoğluFaculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics, Biruni University, İstanbul, Turkey.
Sedef Erkunt AlakEge University Vaccine Development Application and Research Center, İzmir, Turkey.
Muhammet KarakavukEge University Vaccine Development Application and Research Center, İzmir, Turkey.
Aytül GülFaculty of Engineering, Department of Bioengineering, Ege University, İzmir, Turkey.
Mert DöşkayaEge University Vaccine Development Application and Research Center, İzmir, Turkey.
Adnan Yüksel GürüzEge University Vaccine Development Application and Research Center, İzmir, Turkey.
Cemal ÜnFaculty of Science, Department of Biology Molecular Biology Section, Ege University, İzmir, Turkey.
Aysu Değirmenci DöşkayaEge University Vaccine Development Application and Research Center, İzmir, Turkey. aysu.degirmenci.doskaya@ege.edu.tr.
Ege University · TRBiruni University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBartonella henselae is one of the most commonly identified Bartonella species associated with several human diseases. Although B. henselae was detected in humans and cats in Turkey, they have not been genotyped previously. Therefore, this study aimed to genotype B. henselae samples (n = 44) isolated from stray cats using the multi-locus sequence typing (MLST) method. For this aim, eight different housekeeping markers were amplified by nested PCR and then sequenced to reveal sequence types (STs) of B. henselae samples.

resultsAllelic profiles obtained from 40 B. henselae isolates (90.9%) were compatible with available allelic profiles in the MLST online database. However, allelic profiles obtained from the remaining 4 B. henselae isolates (9.1%) were incompatible with the database. Among B. henselae isolates with compatible allelic profiles, 5 different STs including ST1, ST5, ST9, ST35 and ST36 were identified according to the B. henselae MLST online database. ST35 was the most prevalent ST with a prevalence rate of 29.5% (13/44), followed by ST36 with a prevalence rate of 22.7% (10/44). In addition, ST5 (16%, 7/44) and ST9 (18.2%, 8/44) were also among the prevalent STs. The prevalence of ST1 was 4.5% (2/44). For B. henselae isolates with incompatible allelic profiles, we recommended a new ST called ST38.

conclusionThe present study genotyped B. henselae samples isolated from stray cats in Turkey for the first time and ST1, ST5, ST9, ST35, and ST36 as well as a new sequence type named ST38 were identified among these B. henselae isolates.

Indexed as

BartonellaBartonella henselaeBartonella InfectionsCat DiseasesAnimalsCatsDNA, BacterialHumansMultilocus Sequence TypingPolymerase Chain ReactionDNA, BacterialB. henselaeGenetic diversityGenotypingNew sequence type

Identifiers

PMID37805521
PMCPMC10559530
OpenAlexW4387428464

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.