Evidence map›Paper›PMID 37805488›Full record

ArticleBMC complementary medicine and therapies2023

Some new insights into the biological activities of carboxymethylated polysaccharides from Lasiodiplodia theobromae.

Matheus Cerdeira Pires, Natalia de Gois Andriolo, Bruno Rafael Pereira Lopes, Ana Lucia Tasca Gois Ruiz, Valeria Marta Gomes do Nascimento, Karina Alves Toledo, Catarina Dos Santos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary medicine and therapies, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Matheus Cerdeira PiresExperiential Master of Science in Biotechnology, College of Science, Northeastern University, Boston, MA, USA.
Natalia de Gois AndrioloLAQUA (Laboratório de Química da Unesp Assis), University of São Paulo State (UNESP), Assis, SP, Brazil.
Bruno Rafael Pereira LopesLaboratory of Cellular and Molecular Immunology, University of São Paulo State (UNESP), Assis, SP, Brazil.
Ana Lucia Tasca Gois RuizFarmacologia e Toxicologia Experimental), LAFTEx (Laboratório de Fitoquímica, State University of Campinas, Campinas, SP, Brazil.
Valeria Marta Gomes do NascimentoLaboratory of Biochemistry and Bioprocess, University of São Paulo State (UNESP), Assis, SP, Brazil.
Karina Alves ToledoLaboratory of Cellular and Molecular Immunology, University of São Paulo State (UNESP), Assis, SP, Brazil.
Catarina Dos SantosLAQUA (Laboratório de Química da Unesp Assis), University of São Paulo State (UNESP), Assis, SP, Brazil. catarina.santos@unesp.br.
Universidade de São Paulo · BRBoston University · USUniversidade Estadual de Campinas (UNICAMP) · BR

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/20227-8Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/17930-1
6 · The paper itself

Abstract

backgroundCarboxymethylated Lasiodiplodan (LaEPS-C), Lasiodiplodia theobromae β-glucan exopolysaccharide derivative, has a well-known range of biological activities. Compared to LaEPS-C, its fractions, Linear (LLaEPS-C) and Branched (BLaEPS-C), have biological potentialities scarcely described in the literature. So, in this study, we investigate the immunomodulatory, antiviral, antiproliferative, and anticoagulant activities of LLaEPS-C and BLaEPS-C and compare them to the LaEPS-C.

methodsLaEPS was obtained from L. theobromae MMBJ. After carboxymethylation, LaEPS-C structural characteristics were confirmed by Elementary Composition Analysis by Energy Dispersive X-Ray Detector (EDS), Fourier Transform Infrared (FTIR), and Nuclear Magnetic Resonance (NMR). The immunomodulatory activity on cytokine secretion was evaluated in human monocyte-derived macrophage cultures. The antiviral activity was evaluated by Hep-2 cell viability in the presence or absence of hRSV (human respiratory syncytial virus). In vitro antiproliferative activity was tested by sulforhodamine B assay. The anticoagulant activity was determined by APTT (Activated Partial Thromboplastin Time) and PT (Prothrombin Time).

resultsLaEPS-C showed low macrophage cell viability only at 100 µg/mL (52.84 ± 24.06, 48 h), and LLaEPS-C presented no effect. Conversely, BLaEPS-C showed cytotoxicity from 25 to 100 µg/mL (44.36 ± 20.16, 40.64 ± 25.55, 33.87 ± 25.16; 48 h). LaEPS-C and LLaEPS-C showed anti-inflammatory activity. LaEPS-C presented this at 100 µg/mL (36.75 ± 5.53, 48 h) for IL-10, and LLaEPS-C reduces TNF-α cytokine productions at 100 µg/mL (18.27 ± 5.80, 48 h). LLaEPS-C showed an anti-hRSV activity (0.7 µg/ml) plus a low cytotoxic activity for Hep-2 cells (1.4 µg/ml). LaEPS-C presented an antiproliferative activity for NCI-ADR/RES (GI

conclusionsThese findings demonstrated that carboxymethylation effectively improves the biological potential of the LaEPS-C and their fractions. From those polysaccharides tested, LLaEPS provided the best results with low toxicity for anti-inflammatory, antiviral, and anticoagulant activities.

Indexed as

CytokinesPolysaccharidesAnticoagulantsAnti-Inflammatory AgentsAntiviral AgentsAscomycotaHumansAnticoagulantsAnti-Inflammatory AgentsAntiviral AgentsCytokinesPolysaccharidesAnticoagulant activitiesAntiproliferativeImmunomodulatory and AntiviralLasiodiplodan

Identifiers

PMID37805488
PMCPMC10559501
OpenAlexW4387428556

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.