Evidence map›Paper›PMID 37805104›Full record

ArticleDevelopmental biology2023

The BAF chromatin complex component SMARCC1 does not mediate GLI transcriptional repression of Hedgehog target genes in limb buds.

Janani Ramachandran, Wanlu Chen, Rachel K Lex, Kathryn E Windsor, Hyunji Lee, Tingchang Wang, Weiqiang Zhou, Hongkai Ji, Steven A Vokes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Janani RamachandranDepartment of Molecular Biosciences, University of Texas at Austin, 100 E 24th Street, Stop A5000, Austin, TX, 78712, USA.
Wanlu ChenDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
Rachel K LexDivision of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Kathryn E WindsorDepartment of Molecular Biosciences, University of Texas at Austin, 100 E 24th Street, Stop A5000, Austin, TX, 78712, USA.
Hyunji LeeDepartment of Molecular Biosciences, University of Texas at Austin, 100 E 24th Street, Stop A5000, Austin, TX, 78712, USA.
Tingchang WangDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
Weiqiang ZhouDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
Hongkai JiDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD, 21205, USA.
Steven A VokesDepartment of Molecular Biosciences, University of Texas at Austin, 100 E 24th Street, Stop A5000, Austin, TX, 78712, USA. Electronic address: svokes@austin.utexas.edu.
Johns Hopkins University · USThe University of Texas at Austin · USFred Hutch Cancer Center · US

Funding

Cis-regulatory circuitry underlying Hedgehog mediated limb developmentR01HD073151 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI Steven Alexander Vokes · 2012 to 2026
$5.1M
Defining the epigenetic mechanisms underlying GLI-mediated transcriptional repressionF31DE027597 · NIDCR · UNIVERSITY OF TEXAS AT AUSTIN · PI LEX, RACHEL K · 2017 to 2020
$122k
NICHD NIH HHS R01 HD073151NIDCR NIH HHS F31 DE027597
6 · The paper itself

Abstract

Transcriptional responses to the Hedgehog (HH) signaling pathway are primarily modulated by GLI repression in the mouse limb. Previous studies suggested a role for the BAF chromatin remodeling complex in mediating GLI repression. Consistent with this possibility, the core BAF complex protein SMARCC1 is present at most active limb enhancers including the majority of GLI enhancers. However, in contrast to GLI repression which reduces chromatin accessibility, SMARCC1 maintains chromatin accessibility at most enhancers, including those bound by GLI. Moreover, SMARCC1 binding at GLI-regulated enhancers occurs independently of GLI3. Consistent with previous studies, some individual GLI target genes are mis-regulated in Smarcc1 conditional knockouts, though most GLI target genes are unaffected. Moreover, SMARCC1 is not necessary for mediating constitutive GLI repression in HH mutant limb buds. We conclude that SMARCC1 does not mediate GLI3 repression, which we propose utilizes alternative chromatin remodeling complexes.

Indexed as

ChromatinLimb BudsAnimalsHedgehog ProteinsMiceNerve Tissue ProteinsTranscription FactorsZinc Finger Protein GLI1Zinc Finger Protein Gli3ChromatinHedgehog ProteinsNerve Tissue ProteinsSmarcc1 protein, mouseTranscription FactorsZinc Finger Protein GLI1Zinc Finger Protein Gli3BAFGLI3HedgehogLimb budSWI/SNFTranscriptional repression

Identifiers

PMID37805104
PMCPMC11610522
OpenAlexW4387376817

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.