ArticleThe Plant cell2023
Transcriptional activation by WRKY23 and derepression by removal of bHLH041 coordinately establish callus pluripotency in Arabidopsis regeneration.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Time-series transcriptome and metabolome profiling Uncovers WRKY6 and WRKY23 as critical regulators in tobacco response to Pseudomonas syringae infection.Journal of advanced research · 2026Article
- Metabolic control of cellular reprogramming: phospholipid remodeling tunes auxin signaling.The Plant cell · 2026Article
- Revealing the genetic regulation of wood traits and secondary cell wall development inHorticulture research · 2026Article
- The heat is on: hSFA1 drives regeneration after wounding.The Plant cell · 2026Article
- Histone deacetylases and cell-cycle regulators orchestrate cell-identity transitions during Arabidopsis root regeneration.Molecular plant · 2026Article
- TheForestry research · 2026Article
- Cytokinin-supplemented medium drivesFrontiers in plant science · 2026Article
- How tomatoes balance defense: Orchestration of gray mold immunity by the SlMYC2-SlLBD40/42-SlBPM4 module.The Plant cell · 2025Article
- Integrated Multi-Omics Reveals Mechanism of Adventitious Buds Regeneration in In Vitro Cultures ofPlants (Basel, Switzerland) · 2025Article
- Global landscape of protein phosphorylation during plant regeneration initiation in cotton (Gossypium hirsutum L.).BMC biology · 2025Article
- Genome-wide characterization and expression analysis of the bHLH gene family in response to abiotic stresses in Zingiber officinale Roscoe.BMC genomics · 2025Article
- Editorial: Regeneration of plant organsFrontiers in plant science · 2025Article
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Hoxc10-mediated 'positional memory' regulates cartilage formation subsequent to femoral heterotopic grafting.Journal of cellular and molecular medicine · 2024Article
- Proteomic and Phosphoproteomic Analyses during Plant Regeneration Initiation in Cotton (Genes · 2024Article
- A point mutation in thePhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- The roles of epigenetic regulators in plant regeneration: Exploring patterns amidst complex conditions.Plant physiology · 2024Review
- Illumina-based transcriptomic analysis of the fast-growing leguminous treeFrontiers in plant science · 2024Article
- How does light regulate plant regeneration?Frontiers in plant science · 2024Review
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Authors and funding
9 authors at 1 institution in 1 country.
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Abstract
Induction of the pluripotent cell mass termed callus from detached organs or tissues is an initial step in typical in vitro plant regeneration, during which auxin-induced ectopic activation of root stem cell factors is required for subsequent de novo shoot regeneration. While Arabidopsis (Arabidopsis thaliana) AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 and their downstream transcription factors LATERAL ORGAN BOUNDARIES DOMAIN (LBD) are known to play key roles in directing callus formation, the molecules responsible for activation of root stem cell factors and thus establishment of callus pluripotency are unclear. Here, we identified Arabidopsis WRKY23 and BASIC HELIX-LOOP-HELIX 041 (bHLH041) as a transcriptional activator and repressor, respectively, of root stem cell factors during establishment of auxin-induced callus pluripotency. We show that auxin-induced WRKY23 downstream of ARF7 and ARF19 directly activates the transcription of PLETHORA 3 (PLT3) and PLT7 and thus that of the downstream genes PLT1, PLT2, and WUSCHEL-RELATED HOMEOBOX 5 (WOX5), while LBD-induced removal of bHLH041 derepresses the transcription of PLT1, PLT2, and WOX5. We provide evidence that transcriptional activation by WRKY23 and loss of bHLH041-imposed repression act synergistically in conferring shoot-regenerating capability on callus cells. Our findings thus disclose a transcriptional mechanism underlying auxin-induced cellular reprogramming, which, together with previous studies, outlines the molecular framework of auxin-induced pluripotent callus formation for in vitro plant regeneration programs.
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