Evidence map›Paper›PMID 37803605›Full record

ArticleFood research international (Ottawa, Ont.)2023

2'-Fucosyllactose (2'-FL) changes infants gut microbiota composition and their metabolism in a host-free human colonic model.

Shiqi Zhang, Li Chen, Ming Hu, Jiangjiang Zhu

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In one paragraph

Article in Food research international (Ottawa, Ont.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shiqi ZhangHuman Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
Li ChenHuman Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
Ming HuHuman Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
Jiangjiang ZhuHuman Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA; James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA. Electronic address: zhu.2484@osu.edu.

Funding

Characterizing the Metabolome and Volatilome of Gut MicrobiomeR35GM133510 · NIGMS · OHIO STATE UNIVERSITY · PI ZHU, JIANGJIANG · 2019 to 2023
$2.2M
NIGMS NIH HHS R35 GM133510
6 · The paper itself

Abstract

backgroundBreast milk is critical for neonates, providing the necessary energy, nutrients, and bioactive compounds for growth and development. Research indicated that human milk oligosaccharides (HMOs) have been shown to shape a beneficial gut microbiota, as well as their metabolism (e.g. short-chain fatty acids). 2'-Fucosyllactose (2'-FL) is one major HMO that composed of 30% of total HMOs.

objectivesThis study aimed to understand the impact of 2'-FL on the composition and metabolism of infant gut microbiota.

methodsOur study utilized an in-vitro human colonic model (HCM) to investigate the host-free interactions between 2'-FL and infant gut microbiota. To simulate the infant gut microbiota, we inoculated the HCM system with eight representative bacterial species from infant gut microbiota. The effects of 2'-FL on the gut microbial composition and their metabolism were determined through real-time quantitative PCR and liquid-chromatography mass spectrometry (LC/MS). The obtained data were analyzed using Compound Discoverer 3.1 and MetaboAnalyst 4.0.

resultsOur study findings suggest that the intervention of 2'-FL in HCM resulted in a significant change in the abundance of representative bacterial species. PCR analysis showed a consistent increase in the abundance of Parabacteroides. distasonis in all three colon sections. Furthermore, analysis of free fatty acids revealed a significant increase in their levels in the ascending, transverse, and descending colons, except for caproic acid, which was significantly reduced to a non-detectable level. The identification of significant extracellular polar metabolites, such as glutathione and serotonin, enabled us to distinguish between the metabolomes before and after 2'-FL intervention. Moreover, correlation analysis revealed a significant association between the altered microbes and microbial metabolites.

conclusionsIn summary, our study demonstrated the impact of 2'-FL intervention on the defined composition of infant gut microbiota and their metabolic pathways in an in vitro setting. Our findings provide valuable insights for future follow-up investigations into the role of 2'-FL in regulating the growth and development of infant gut microbiota in vivo.

Indexed as

Gastrointestinal MicrobiomeColonFemaleHumansInfantInfant, NewbornMilk, HumanOligosaccharidesTrisaccharides2'-fucosyllactoseOligosaccharidesTrisaccharides2′-FLGut microbiotaHuman milk oligosaccharideInfantMetabolomics

Identifiers

PMID37803605
PMCPMC10560763

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.