ArticleJournal of dental sciences2023
Evaluation of extracellular polymeric substances matrix volume, surface roughness and bacterial adhesion property of oral biofilm.
Article in Journal of dental sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 37 citations in OpenAlex.
- Trial
- Biofilm Dynamics and Antimicrobial Resistance in Rabbit Odontogenic Infections: A One Health Perspective.Pathogens (Basel, Switzerland) · 2026Review
- Optical Attenuation Analysis of Biofilm Formation on Dental Prostheses Using Time-Domain Optical Coherence Tomography and Atomic Force Microscopy.Dentistry journal · 2026Article
- Review
- Beyond the Conventional: Successful Negotiation and Management of the Middle Mesial Canal.Cureus · 2026Article
- Adaptive remodeling of cell wall peptidoglycan architecture inFood chemistry: X · 2026Article
- Polymethyl methacrylate-hydroxyapatite: A viable non-metallic alternative for dental implants? Comprehensive surface characterization.Journal of Taibah University Medical Sciences · 2026Article
- The impact of DNA extraction methods on species quantification and apparent community composition of in vitro oral biofilms.FEMS microbiology letters · 2026Article
- Stretch regulated surface roughness controls macrophage inflammatory polarization through adhesion-chromatin coupling.Frontiers in pharmacology · 2026Article
- Cellulose Hydrogels Derived from Pineapple Bagasse for Potential Dental Applications: Chlorhexidine-Loaded Hydrogels with Antibacterial and Cytocompatible Properties.Gels (Basel, Switzerland) · 2025Article
- Effect of 6-Shogaol Derived from Ginger (Nutrients · 2025Article
- The impact of different mechanical file systems with or without adjunctive approaches on disinfection of oval root canals: an ex vivo study.British dental journal · 2025Article
- Evaluation of bacterial biofilm, smear layer, and debris removal efficacy of a hydro-dynamic cavitation system with physiological saline using a new ex vivo model: a CLSM and SEM study.BMC oral health · 2025Article
- Article
- Article
- Endodontic Treatment of a Maxillary Second Molar With Five Canals: A Case Report and a Literature Review.Cureus · 2024Article
- Article
- Endodontic Retreatment of a Mandibular Second Molar With a C-shaped Root Canal Configuration: A Case Report.Cureus · 2024Article
- Article
- Characterization and morphological methods for oral biofilm visualization: where are we nowadays?AIMS microbiology · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background/purpose: Oral biofilms are highly structured bacterial colonies embedded in a highly hydrated extracellular polymeric substances (EPS) matrix. This study aimed to investigate the characteristics of oral biofilm at different stages of maturation. Materials and methods: Oral multispecies biofilms were grown anaerobically from plaque bacteria on collagen coated hydroxyapatite discs in brain heart infusion broth for one and three weeks. The volume of live bacteria and EPS matrix of the biofilms were determined by using corresponding fluorescent probes and confocal laser scanning microscopy. Atomic force microscopy (AFM) was used to quantitatively probe and correlate cell surface adhesion force of biofilms. The surface roughness was quantified in terms of the root mean square average of the height deviations. Adhesion was measured from force-distance data for the retraction of the cell from the surface. Results: The volume of live bacteria and EPS of 3-week-old biofilms was higher than 1-week-old biofilms. The surface roughness value in 1-week-old biofilms was significantly higher than that in 3-week-old biofilms. AFM force-distance curve results showed that the adhesion force at the cell-cell interface was significantly more at-tractive than those at bacterial cells surface of both stages biofilms. Adhesion forces between the AFM tip and the surface of bacterial cell were fairly constant, whereas the cell-cell interface experienced greater adhesion forces in the biofilm's development. Conclusion: As oral biofilms become mature, EPS volume and cell-cell adhesion forces increase while the surface roughness decreases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.