ArticleAnnals of clinical microbiology and antimicrobials2023
Effect of ZnO nanoparticles on biofilm formation and gene expression of the toxin-antitoxin system in clinical isolates of Pseudomonas aeruginosa.
Article in Annals of clinical microbiology and antimicrobials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 35 citations in OpenAlex.
- Mn Doping Enhances the Antibacterial, Antibiofilm and Anti-Virulence Activity of ZnO Nanoparticles.Nanomaterials (Basel, Switzerland) · 2026Article
- Synthesis of zinc oxide nanoparticles and their effect on biofilm of methicillin-resistant Staphylococcus aureus isolates.BMC microbiology · 2026Article
- Antimicrobial Systems Based on Essential Oils Advanced by Zinc Oxide Nanoparticles.ACS pharmacology & translational science · 2026Review
- Multiple virulence attenuating strategies against Pseudomonas aeruginosa: Natural, synthetic, and synergistic approaches.World journal of microbiology & biotechnology · 2026Review
- Mapping the knowledge landscape ofFrontiers in cellular and infection microbiology · 2026Review
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- Ni-Fe Nanoparticles fromCurrent pharmaceutical design · 2026Article
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- Neem (Azadirachta indica) leaf extract mediated synthesis of zinc oxide nanoparticles (ZnO NPs) and their antibacterial activity.Discover nano · 2025Article
- Effect of Zinc and Magnesium Compounds and Nano-Hydroxyapatite on the Physicochemical Properties and Biological Activity of Alginate and Gelatin Scaffolds for Osteochondral Defects.Journal of functional biomaterials · 2025Article
- Functionalization of gelatin films dressings with silver-doped zinc oxide nanoparticles for prevention of bacterial infection and promoting wound repair.Scientific reports · 2025Article
- Metal-Based Approaches for the Fight against Antimicrobial Resistance: Mechanisms, Opportunities, and Challenges.Journal of the American Chemical Society · 2025Review
- HarnessingNanomaterials (Basel, Switzerland) · 2025Article
- Eco-friendly zinc oxide nanoparticle biosynthesis powered by probiotic bacteria.Applied microbiology and biotechnology · 2025Review
- Zinc oxide nanoparticles as a new approach to eradicate the biofilm ofCaspian journal of internal medicine · 2025Article
- Developing a Urinary Catheter with Anti-Biofilm Coated Surface Using Phyto-Assisted Synthesis of Zinc Oxide Nanoparticles.Infection and drug resistance · 2025Article
- Low-dose zinc oxide nanoparticles trigger the growth and biofilm formation of Pseudomonas aeruginosa: a hormetic response.BMC microbiology · 2024Article
- Synthesis and characterization of hydroxyapatite-zinc oxide nanocomposites incorporating rosemary and thyme essential oils for enhanced bone regeneration and antimicrobial activity.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologieArticle
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBiofilm formation by Pseudomonas aeruginosa (P. aeruginosa) is known to be characteristic of this organism. This bacterium is considered one of the most life-threatening bacteria and has been identified as a priority pathogen for research by WHO. Biofilm-producing P. aeruginosa is a concern in many parts of the world due to antibiotic resistance. Alginate also plays an important role in the biofilm formation of P. aeruginosa as well as the emergence of antibiotic resistance in biofilms. In addition, the systems of toxin-antitoxin( TA) play an important role in biofilm formation. Metal nanoparticle(NP) such as zinc oxide (ZnO) also have extensive biological properties, especially anti-biofilm properties. Therefore, this study was conducted in relation to the importance of zinc oxide nanoparticles (ZnO NPs) in biofilm formation and also the correlation of gene expression of TA systems in clinical isolates of P. aeruginosa.
methodsA total of 52 P. aeruginosa isolates were collected from burns (n = 15), UTI (n = 31), and trachea (n = 6) in hospitals in Ilam between May 2020 and October 2020. Biofilm formation was assessed using a microtiter plate assay. MIC and sub-MIC concentrations of ZnO NPs (10-30 nm with purity greater than 99.8%) in P. aeruginosa were determined. Subsequently, biofilm formation was investigated using sub-MIC concentrations of ZnO NPs. Finally, total RNA was extracted and RT- qPCR was used to determine the expression levels of genes of mazEF, mqsRA, and higBA of TA systems.
resultsSix isolates of P. aeruginosa were found to form strong biofilms. The results showed that ZnO NPs were able to inhibit biofilm formation. In our experiments, we found that the sub-MIC concentration of ZnO NPs increased the gene expression of antitoxins mazE and mqsA and toxin higB of TA systems treated with ZnO NPs.
conclusionsIn the present study, ZnO NPs were shown to effectively inhibit biofilm formation in P. aeruginosa. Our results support the relationship between TA systems and ZnO NPs in biofilm formation in P. aeruginosa. Importantly, the expression of antitoxins mazE and mqsA was high after treatment with ZnO NPs, but not that of antitoxin higA.
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