ArticlePlant biotechnology journal2024
The miR156/SPL12 module orchestrates fruit colour change through directly regulating ethylene production pathway in blueberry.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Anthocyanin biosynthesis in blueberry is regulated by light and abscisic acid signaling via a VcABF2-miR156-VcSPL9 feedback loop.The New phytologist · 2026Article
- miR156-StSPL9 regulating the biosynthesis of flavonoids and secondary metabolites in potato tubers.BMC plant biology · 2026Article
- MicroRNA-Mediated Hormonal Control of Fruit Morphology.Plants (Basel, Switzerland) · 2026Review
- Regulatory mechanisms and multidimensional applications of blueberry anthocyanins: a review of current gaps and future directions.Engineering in life sciences · 2026Review
- Genome-wide identification of the SBP-box gene family in banana and functional characterization of MaSBP17 as a negative regulator of fruit ripening.Frontiers in plant science · 2026Article
- GhmiR156-International journal of molecular sciences · 2025Article
- Post-transcriptional and post-translational regulation of anthocyanin biosynthesis in sweetpotato by Ib-miR2111 and IbKFB: Implications for health promotion.Journal of advanced research · 2025Article
- A Repression-Activation System Involving miR164a-NACs Regulates Tissue-Specific Anthocyanin Accumulation in Peach Fruit.Plant biotechnology journal · 2025Article
- Evolutionary Insights and Flowering Regulation ofPlants (Basel, Switzerland) · 2025Article
- Blueberry ripening mechanism: a systematic review of physiological and molecular evidence.Horticulture research · 2025Article
- Slow and steady wins the race: the negative regulators of ethylene biosynthesis in horticultural plants.Horticulture research · 2025Article
- Molecular mechanisms driving the unusual pigmentation shift during eggplant fruit development.Plant communications · 2025Article
- Impact of shorter picking intervals on the storability and postharvest quality of rabbiteye blueberries cv. 'Brightwell'.Frontiers in plant science · 2025Article
- The SBP-box transcription factor PlSPL2 negatively regulates stem development in herbaceous peony.Plant cell reports · 2024Article
- The Ethylene Biosynthetic Enzymes, 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase (ACS) and ACC Oxidase (ACO): The Less Explored Players in Abiotic Stress Tolerance.Biomolecules · 2024Review
- The ncRNAs Involved in the Regulation of Abiotic Stress-Induced Anthocyanin Biosynthesis in Plants.Antioxidants (Basel, Switzerland) · 2023Review
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Colour change is an important event during fruit ripening in blueberry. It is well known that miR156/SPLs act as regulatory modules mediating anthocyanin biosynthesis and ethylene plays critical roles during colour change, but the intrinsic connections between the two pathways remain poorly understood. Previously, we demonstrated that blueberry VcMIR156a/VcSPL12 affects the accumulation of anthocyanins and chlorophylls in tomato and Arabidopsis. In this study, we first showed that VcMIR156a overexpression in blueberry led to enhanced anthocyanin biosynthesis, decreased chlorophyll accumulation, and, intriguingly, concomitant elevation in the expression of ethylene biosynthesis genes and the level of the ethylene precursor ACC. Conversely, VcSPL12 enhanced chlorophyll accumulation and suppressed anthocyanin biosynthesis and ACC synthesis in fruits. Moreover, the treatment with ethylene substitutes and inhibitors attenuated the effects of VcMIR156a and VcSPL12 on pigment accumulation. Protein-DNA interaction assays indicated that VcSPL12 could specifically bind to the promoters and inhibit the activities of the ethylene biosynthetic genes VcACS1 and VcACO6. Collectively, our results show that VcMIR156a/VcSPL12 alters ethylene production through targeting VcACS1 and VcACO6, therefore governing fruit colour change. Additionally, VcSPL12 may directly interact with the promoter region of the chlorophyll biosynthetic gene VcDVR, thereby activating its expression. These findings established an intrinsic connection between the miR156/SPL regulatory module and ethylene pathway.
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