ArticleACS synthetic biology2023
Molecular Imaging with Aquaporin-Based Reporter Genes: Quantitative Considerations from Monte Carlo Diffusion Simulations.
Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- A programmable genetic platform for engineering noninvasive biosensors.Science advances · 2026Article
- Motion and Flow Robust Free-Breathing Diffusion Kurtosis Imaging of the Kidney.NMR in biomedicine · 2025Article
- Generation of hTERT-immortalized human mesenchymal stromal cells with optical and magnetic labels for in vivo transplantation and tracking.Stem cell research & therapy · 2025Article
- A programmable genetic platform for engineering noninvasive biosensors.bioRxiv : the preprint server for biology · 2025Article
- Exploring the potential of water channels for developing genetically encoded reporters and biosensors for diffusion-weighted MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- A Dual-Gene Reporter-Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange.Chembiochem : a European journal of chemical biology · 2024Article
- A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange.bioRxiv : the preprint server for biology · 2024Article
- Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents.bioRxiv : the preprint server for biology · 2024Article
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7 authors at 3 institutions in 2 countries.
Funding
Abstract
Aquaporins provide a unique approach for imaging genetic activity in deep tissues by increasing the rate of cellular water diffusion, which generates a magnetic resonance contrast. However, distinguishing aquaporin signals from the tissue background is challenging because water diffusion is influenced by structural factors, such as cell size and packing density. Here, we developed a Monte Carlo model to analyze how cell radius and intracellular volume fraction quantitatively affect aquaporin signals. We demonstrated that a differential imaging approach based on subtracting signals at two diffusion times can improve specificity by unambiguously isolating aquaporin signals from the tissue background. We further used Monte Carlo simulations to analyze the connection between diffusivity and the percentage of cells engineered to express aquaporin and established a mapping that accurately determined the volume fraction of aquaporin-expressing cells in mixed populations. The quantitative framework developed in this study will enable a broad range of applications in biomedical synthetic biology, requiring the use of aquaporins to noninvasively monitor the location and function of genetically engineered devices in live animals.
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Registered trials
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