Evidence map›Paper›PMID 37792779›Full record

ArticleBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica2023

Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone.

E I T de Assis, V A N Azevedo, M F de Lima Neto, F C Costa, L R F M Paulino, P A A Barroso, M H T Matos, A P O do Monte, M A M Donato, C A Peixoto and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

E I T de AssisLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0001-8164-3937
V A N AzevedoLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0003-3194-8913
M F de Lima NetoLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0003-0034-0606
F C CostaLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-7045-1523
L R F M PaulinoLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-4750-3436
P A A BarrosoLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-0243-7471
M H T MatosNúcleo de Biotecnologia Aplicada ao Desenvolvimento do Folículo Ovariano, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brasil.ORCID http://orcid.org/0000-0001-6215-5292
A P O do MonteNúcleo de Biotecnologia Aplicada ao Desenvolvimento do Folículo Ovariano, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brasil.ORCID http://orcid.org/0000-0001-9945-4552
M A M DonatoLaboratório de Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (CPqAM)/FIOCRUZ, Universidade Federal de Pernambuco, Recife, PE, Brasil.ORCID http://orcid.org/0000-0002-3361-2789
C A PeixotoLaboratório de Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (CPqAM)/FIOCRUZ, Universidade Federal de Pernambuco, Recife, PE, Brasil.ORCID http://orcid.org/0000-0002-7008-5930
A N GodinhoNúcleo de Pesquisa em Experimentação Animal, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0003-3410-2315
J M O FreireNúcleo de Pesquisa em Experimentação Animal, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-3412-8964
A L P SouzaLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-0348-4026
J R V SilvaLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0002-5970-6177
A W B SilvaLaboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.ORCID http://orcid.org/0000-0003-4105-426X
Universidade Federal do Ceará · BRUniversidade Federal de Pernambuco · BRUniversidade Federal do Vale do São Francisco · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA. The ovaries were cultured at 37.5°C in 5% CO2 for 6 days. Ovarian morphology, follicular ultrastructure, and the levels of mRNA for Bax, Bcl-2, and Caspase-3 were evaluated. The results showed that DEXA reduced the percentage of morphologically normal follicles, while CIMI prevented the deleterious effects caused by DEXA. In addition, DEXA negatively affected the stromal cellular density, while CIMI prevented these adverse effects. Ovaries cultured with DEXA and CIMI showed similar levels of mRNA for Bax, Bcl-2, and Caspase-3 compared to those cultured in control medium, while ovaries cultured with DEXA had increased expression of the above genes. Additionally, the ultrastructure of the ovaries cultured with CIMI was well preserved. Thus, the extract of CIMI was able to prevent the deleterious effects caused by DEXA on cultured mouse ovaries.

Indexed as

CimicifugaAnimalsbcl-2-Associated X ProteinCaspase 3DexamethasoneFemaleMiceOvarian FollicleProto-Oncogene Proteins c-bcl-2RNA, Messengerbcl-2-Associated X ProteinCaspase 3DexamethasoneProto-Oncogene Proteins c-bcl-2RNA, Messenger

Identifiers

PMID37792779
PMCPMC10515502
OpenAlexW4386973358

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.