Evidence map›Paper›PMID 37792259›Full record

ArticleMolecular neurobiology2024

Identification of Regulatory Elements in Primary Sensory Neurons Involved in Trauma-Induced Neuropathic Pain.

Kimberly E Stephens, Cedric Moore, David A Vinson, Bryan E White, Zachary Renfro, Weiqiang Zhou, Zhicheng Ji, Hongkai Ji, Heng Zhu, Yun Guan and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular neurobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 70% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kimberly E Stephens *Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA. kestephens@uams.edu.ORCID http://orcid.org/0000-0002-8822-0547
Cedric MooreDepartment of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
David A VinsonDepartment of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Bryan E WhiteDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Zachary RenfroDepartment of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Weiqiang ZhouDepartment of Biostatistics, School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Zhicheng JiDepartment of Biostatistics, School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Hongkai JiDepartment of Biostatistics, School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Heng ZhuDepartment of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Yun GuanDepartment of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. yguan1@jhmi.edu.
Sean D TavernaDepartment of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA. staverna@jhmi.edu.
Johns Hopkins University · USArkansas Children's Hospital · US

Funding

The Chemistry-Biology Interface Program at Johns Hopkins UniversityT32GM080189 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ROKITA, STEVEN E · 2008 to 2022
$3.5M
The Chemistry-Biology Interface Program at Johns Hopkins UniversityT32GM149382 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI STEVEN E ROKITA · 2023 to 2026
$1.4M
NIGMS NIH HHS T32 GM080189NIGMS NIH HHS T32 GM149382
6 · The paper itself

Abstract

Chronic pain is a significant public health issue that is often refractory to existing therapies. Here we use a multiomic approach to identify cis-regulatory elements that show differential chromatin accessibility and reveal transcription factor (TF) binding motifs with functional regulation in the rat dorsal root ganglion (DRG), which contain cell bodies of primary sensory neurons, after nerve injury. We integrated RNA-seq to understand how differential chromatin accessibility after nerve injury may influence gene expression. Using TF protein arrays and chromatin immunoprecipitation-qPCR, we confirmed C/EBPγ binding to a differentially accessible sequence and used RNA-seq to identify processes in which C/EBPγ plays an important role. Our findings offer insights into TF motifs that are associated with chronic pain. These data show how interactions between chromatin landscapes and TF expression patterns may work together to determine gene expression programs in rat DRG neurons after nerve injury.

Indexed as

Chronic PainNeuralgiaAnimalsChromatinGanglia, SpinalRatsRats, Sprague-DawleySensory Receptor CellsChromatinCEBPGChromatin accessibilityChronic constriction injuryDorsal root ganglionEpigeneticsNeuropathic pain

Identifiers

PMID37792259
PMCPMC10896855
OpenAlexW4387326469

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.