ArticleBiomedical optics express2023
Imaging and component analysis of pumpkin stem tissue with simultaneous SF-CARS and TPEF microscopy.
Article in Biomedical optics express, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
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Abstract
A multimodal nonlinear optical imaging platform based on a single femtosecond oscillator is built for simultaneous TPEF and SF-CARS imaging. TPEF microscopy and SF-CARS microscopy is utilized for mapping the distribution of the lignin component and the polysaccharide component, respectively. Visualization of vessel structure is realized. And the relative distribution of lignin and polysaccharide of vessel structure is mapped. Two pumpkin stem tissue areas with different degrees of lignification are observed with simultaneous TPEF and SF-CARS imaging, and two types of cell walls are identified. The different distribution patterns of lignin and polysaccharide in these two types of cell walls, induced by different degrees of lignification, are analyzed in detail.
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