Evidence map›Paper›PMID 37790421›Full record

ArticlebioRxiv : the preprint server for biology2023

Structural basis of transcription: RNA Polymerase II substrate binding and metal coordination at 3.0 Å using a free-electron laser.

Guowu Lin, Christopher O Barnes, Simon Weiss, Bercem Dutagaci, Chenxi Qiu, Michael Feig, Jihnu Song, Artem Lyubimov, Aina E Cohen, Craig D Kaplan and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Guowu LinDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh PA 15261 USA.
Christopher O BarnesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena CA 91125 USA.
Simon WeissDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh PA 15261 USA.
Bercem DutagaciDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing MI 48824 USA.
Chenxi QiuDepartment of Genetics, Harvard Medical School, Boston MA 02115 USA.
Michael FeigDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing MI 48824 USA.
Jihnu SongStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Artem LyubimovStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Aina E CohenStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Craig D KaplanDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh PA 15260 USA.
Guillermo CaleroDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh PA 15261 USA.
University of Pittsburgh · USSLAC National Accelerator Laboratory · USMichigan State University · USCalifornia Institute of Technology · USHarvard University · US

Funding

Mechanisms of RNA Polymerase II transcriptionR35GM144116 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Craig Kaplan · 2022 to 2026
$3.4M
Mechanism and Regulation of RNA Polymerase II ElongationR01GM097260 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KAPLAN, CRAIG · 2011 to 2020
$3.3M
Modeling and dynamics of biomolecules on cellular scalesR35GM126948 · NIGMS · MICHIGAN STATE UNIVERSITY · PI Michael Feig · 2018 to 2026
$3.2M
Structural Studies of RNA Polymerase II Transcription Initiation and ElongationR01GM112686 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CALERO, GUILLERMO ALBERTO · 2015 to 2024
$2.8M
NIGMS NIH HHS R01 GM097260NIGMS NIH HHS R01 GM112686NIGMS NIH HHS R35 GM126948NIGMS NIH HHS R35 GM144116
6 · The paper itself

Abstract

Catalysis and translocation of multi-subunit DNA-directed RNA polymerases underlie all cellular mRNA synthesis. RNA polymerase II (Pol II) synthesizes eukaryotic pre-mRNAs from a DNA template strand buried in its active site. Structural details of catalysis at near atomic resolution and precise arrangement of key active site components have been elusive. Here we present the free electron laser (FEL) structure of a matched ATP-bound Pol II, revealing the full active site interaction network at the highest resolution to date, including the trigger loop (TL) in the closed conformation, bonafide occupancy of both site A and B Mg

Identifiers

PMID37790421
PMCPMC10543002
OpenAlexW4386982464

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.