Evidence map›Paper›PMID 37787784›Full record

ArticleNaunyn-Schmiedeberg's archives of pharmacology2024

Anti-proliferative activity of chitosan-coated oxypeucedanin nano-chitosomes (COPD-NCs) against human HT-29 colon cancer cells: in vitro study.

Muntadher Aqeel Obaid Almohammed, Sakineh Meshkani, Masoud Homayouni Tabrizi, Mahshid Sharbatiyan, Hasti Nasiraei Haghighi

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 65% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Muntadher Aqeel Obaid AlmohammedPlasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Sakineh MeshkaniPlasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran. s.meshkani@gmail.com.
Masoud Homayouni TabriziDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Mahshid SharbatiyanDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Hasti Nasiraei HaghighiDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Islamic Azad University, Mashhad · IRIslamic Azad University, Science and Research Branch · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxypeucedanin (OPD) as a powerful anti-proliferative agent found in the Angelicae dahuricae has been used to suppress cancer cell growth. However, the hydrophobic chemical structure has limited its solubility and bio-accessibility. This is the first time OPD is encapsulated into a nano-liposomal structure and coated with poly-cationic chitosan polymer as the oxypeucedanin drug delivery system to evaluate its antioxidant and anti-colon cancer potential. The chitosan-coated oxypeucedanin nano-chitosomes (COPD-NCs) were synthesized utilizing the thin-layer hydration method and characterized by FESEM, DLS, FTIR, and zeta potential analysis. The anti-cancer potential of COPD-NC was analyzed by measuring the cell survival rate (MTT assay) and studying the cellular death type (AO/PI staining) following the increased treatment concentrations of COPD-NC on the HT-29 colon cancer cell line. Moreover, the COPD-NCs' apoptotic activity was verified by analyzing Cas-3 and Cas-9 gene expression profiles. Finally, the COPD-NCs' antioxidant activity was evaluated by applying ABTS, DPPH, and FRAP antioxidant assays. The 258.26-nm COPD-NCs significantly inhibited the HT-29 colon cancer cells compared with the normal fibroblast HFF cells. The up-regulated Cas-3 and Cas-9 gene expression exhibited the COPD-NCs' apoptotic activity. Also, the COPD-NCs' apoptotic activity was verified by detecting the increased apoptotic bodies following the AO/PI fluorescent staining in the increased exposure doses of COPD-NCs. Ultimately, the COPD-NCs meaningfully inhibited the ABTS-DPPH radicals and exhibited an appropriate FRAP-reductive potential. The designed nanostructure for COPD-NCs significantly improved its antioxidant potential and selective cytotoxicity on human HT-29 human cancer cells, which makes them a safe selective natural drug delivery system. Therefore, the COPD-NCs can selectively induce apoptotic death in human HT-29 cancer cells and have the potential to be studied as an anti-colon cancer compound. However, further cancer and normal cell lines are required to verify their selective cytotoxicity.

Indexed as

BenzothiazolesChitosanColonic NeoplasmsFurocoumarinsPulmonary Disease, Chronic ObstructiveSulfonic AcidsAntioxidantsHT29 CellsHumans2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acidAntioxidantsBenzothiazolesChitosanFurocoumarinsoxypeucadaninSulfonic AcidsAnti-colon cancer compoundAntioxidantAnti-proliferativeApoptotic activityChitosan-coated oxypeucedanin nano-chitosome (COPD-NC)Oxypeucedanin drug delivery systemSelective cytotoxicity

Identifiers

PMID37787784
OpenAlexW4387296531

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.