Evidence map›Paper›PMID 37786702›Full record

ArticlebioRxiv : the preprint server for biology2023

Label-free, real-time monitoring of membrane binding events at zeptomolar concentrations using frequency-locked optical microresonators.

Adley Gin, Phuong-Diem Nguyen, Jeffrey E Melzer, Cheng Li, Hannah Strzelinski, Stephen B Liggett, Judith Su

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Adley GinWyant College of Optical Sciences, The University of Arizona, Tucson, AZ 85721.
Phuong-Diem NguyenDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721.
Jeffrey E MelzerWyant College of Optical Sciences, The University of Arizona, Tucson, AZ 85721.
Cheng LiWyant College of Optical Sciences, The University of Arizona, Tucson, AZ 85721.
Hannah StrzelinskiDepartment of Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612.
Stephen B LiggettDepartment of Medicine, University of South Florida Morsani College of Medicine, Tampa, FL 33612.
Judith SuWyant College of Optical Sciences, The University of Arizona, Tucson, AZ 85721.
University of Arizona · USUniversity of South Florida · US

Funding

Project 4 - OGR1- and TSPO- dependent mechanisms mediated by benzodiazepines affecting ASM contractionP01HL114471 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI LIGGETT, STEPHEN B · 2013 to 2023
$23.9M
Characterization of biased airway smooth muscle TAS2R agonists for treating asthmaR01HL155532 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI LIGGETT, STEPHEN B · 2021 to 2024
$2.2M
Label-free single molecule detection for basic science and translational medicineR35GM137988 · NIGMS · UNIVERSITY OF ARIZONA · PI SU, JUDITH · 2020 to 2024
$1.8M
Label-free, highly-specific, small molecule detection using microtoroid optical resonatorsR21MH111109 · NIMH · UNIVERSITY OF ARIZONA · PI SU, JUDITH · 2016 to 2017
$396k
NHLBI NIH HHS P01 HL114471NHLBI NIH HHS R01 HL155532NIGMS NIH HHS R35 GM137988NIMH NIH HHS R21 MH111109
6 · The paper itself

Abstract

Binding events to elements of the cell membrane act as receptors which regulate cellular function and communication and are the targets of many small molecule drug discovery efforts for agonists and antagonists. Conventional techniques to probe these interactions generally require labels and large amounts of receptor to achieve satisfactory sensitivity. Whispering gallery mode microtoroid optical resonators have demonstrated sensitivity to detect single-molecule binding events. Here, we demonstrate the use of frequency-locked optical microtoroids for characterization of membrane interactions

Identifiers

PMID37786702
PMCPMC10541581
OpenAlexW4386946531

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.