Quantification of soluble very low-density lipoprotein receptor in human serum using a sandwich enzyme-linked immunosorbent assay.

Yoshiharu Tokita, Kazuya Miyashita, Katsuyuki Nakajima, Sadao Takahashi, Akira Tanaka

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In one paragraph

Article in Practical laboratory medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact, top 52% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Yoshiharu TokitaFaculty of Medical Technology and Clinical Engineering, Gunma University of Health and Welfare, Gunma, Japan.

Kazuya MiyashitaImmuno-biological Laboratories Co, Gunma, Japan.

Katsuyuki NakajimaDepartment of Clinical Laboratory Medicine, Gunma University, Graduate School of Medicine, Gunma, Japan.

Sadao TakahashiKagawa Nutrition University, Tokyo, Japan.

Akira TanakaKichijoji Futaba Professional and Vocational College of Culinary Nutrition, Tokyo, Japan.

Kagawa Nutrition University · JPFutaba (Japan) · JPGunma University · JPMedical & Biological Laboratories (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To investigate the regulation of soluble very low-density lipoprotein receptor (sVLDL-R), which is cleaved mostly from the extracellular domain of VLDL-R II, we generated two rat monoclonal antibodies (mAbs) against human sVLDL-R, and used them to develop a sandwich enzyme-linked immunosorbent assay (ELISA) to measure sVLDL-R levels in human serum or plasma. The ELISA had a linear range from 0.20 ng/mL to 13.02 ng/mL and allowed for the quantification of sVLDL-R in serum and culture cell medium. The coefficient of variation (CV) was less than 10% for both the intra- and inter-assays. The bililubin F, and C, triglyceride (TG), and hemoglobin levels did not affect assay precision. The sVLDL-R concentration was negatively associated with body fat percentage, TG, and HbA1c, suggesting the possibility of obesity and diabetes in middle-aged Japanese women.

Indexed as

Body fat percentageHbA1cSandwich enzyme-linked immunosorbent assay (ELISA)Soluble very low density lipoprotein receptor (sVLDL-R)Triglyceride (TG)

Identifiers

PMID37781344

DOI10.1016/j.plabm.2023.e00337

PMCPMC10539655

OpenAlexW4386914136

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.