Evidence map›Paper›PMID 37780843›Full record

ArticleFrontiers in cellular and infection microbiology2023

Modifying

Guangyuan Zhang, Jia Liu, Yonglin He, Yuheng Du, Lei Xu, Tingting Chen, Yanan Guo, Huichao Fu, Anlong Li, Yunbo Tian and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Guangyuan ZhangChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Jia LiuChongqing Medical University, Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing, China.
Yonglin HeChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Yuheng DuDepartment for Rehabilitation Medicine, The Second College of Clinical Medicine of Chongqing Medical University, Chongqing, China.
Lei XuChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Tingting ChenChongqing Medical University, Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing, China.
Yanan GuoChongqing Medical University, International Medical College, Chongqing, China.
Huichao FuChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Anlong LiChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Yunbo TianChongqing Blood Center, Quality Management Section, Chongqing, China.
Yan HuTuberculosis Reference Laboratory, Chongqing Tuberculosis Control Institute, Chongqing, China.
Chun YangChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Mingqi LuChongqing Medical University, Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing, China.
Xichuan DengChongqing Medical University, Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing, China.
Jingsong WangR&D Department, Chongqing Kebilong Biotechnology Co., Ltd., Chongqing, China.
Nan LuChongqing Medical University, Basic Medical School, Department of Pathogen Biology, Chongqing, China.
Chongqing Medical University · CNCenter for Tuberculosis Control of Guangdong Province · CNDalian Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Laboratory teaching of medical microbiology involves highly pathogenic microorganisms, thus posing potential biosafety risks to the students and the teacher. To address these risks, non/low-pathogenic microorganisms were modified to mimic highly pathogenic ones or highly pathogenic microorganisms were attenuated directly using the CRISPR/Cas9 technology. This study describes the modification of Methods: To generate Results: The survey revealed that most teachers and students were inclined towards real-time laboratory classes than virtual classes or observation of plastic specimens. However, many students did not abide by the safety regulations, and most encountered potential biosafety hazards in the laboratory. Discussion: This approach can be applied to other high-risk pathogenic microorganisms to reduce the potential biosafety risks in medical laboratory-based teaching and provide a new strategy for the development of experimental materials.

Indexed as

Escherichia coliShigellaAnimalsContainment of BiohazardsGuinea PigsHumansShigella flexneriVirulencebiosafetyEscherichia coli DH5αmedical microbiology laboratory class teachingShigellasubstitution materials

Identifiers

PMID37780843
PMCPMC10533986
OpenAlexW4386699009

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.