ArticleChembiochem : a European journal of chemical biology2023
Stereoselective Biocatalytic α-Deuteration of L-Amino Acids by a Pyridoxal 5'-Phosphate-Dependent Mannich Cyclase.
Article in Chembiochem : a European journal of chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Chemotypic diversity of the fungusApplied and environmental microbiology · 2026Article
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- A pyridoxal phosphate-dependent enzyme platform for stereoselective synthesis of γ-tertiary-nitro-noncanonical amino acids.Nature communications · 2026Article
- Emerging enzymatic strategies for the synthesis of α-tertiary amino acids.Communications chemistry · 2026Review
- Intracellular assembly of artificial enzymes for cytoplasmic enantioselective Mannich reactions.Nature communications · 2026Article
- Stereoselective hydrogen isotope exchange on nicotinamide cofactors through flavoenzyme microscopic reversibility.Chemical science · 2026Article
- Radiolabeling of oligopeptides by selective hydrogen isotope exchange with deuterium and tritium in aqueous buffers.Nature communications · 2026Article
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- Highly Sensitive Detection of Tyrosine and Neurotransmitters by Stereoselective Biosynthesis and Photochemically Induced Dynamic Nuclear Polarization.Journal of the American Chemical Society · 2025Article
- Diversity-oriented photobiocatalytic synthesis via stereoselective three-component radical coupling.Science (New York, N.Y.) · 2025Article
- Development of the Direct Deuteration Method for Amino Acids and Characterization of Deuterated Tryptophan.Bioengineering (Basel, Switzerland) · 2025Article
- Molecular basis for the diversification of lincosamide biosynthesis by pyridoxal phosphate-dependent enzymes.Nature chemistry · 2025Article
- Nature-Inspired Radical Pyridoxal-Mediated C-C Bond Formation.Journal of the American Chemical Society · 2024Article
- Enzymatic Synthesis of Unprotected α,β-Diamino Acids via Direct Asymmetric Mannich Reactions.Journal of the American Chemical Society · 2024Article
- Article
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3 authors.
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Abstract
α-Deuterated amino acids are valuable building blocks for developing deuterated drugs, and are important tools for studying biological systems. Biocatalytic deuteration represents an attractive strategy to directly access enantiopure α-deuterated amino acids. Here, we show that a PLP-dependent Mannich cyclase, LolT, involved in the biosynthesis of loline alkaloids, is capable of deuterating a diverse range of L-amino acids, including basic and acidic, nonpolar and polar, aliphatic and aromatic amino acids. Furthermore, complete deuteration of many amino acids can be achieved within minutes with exquisite control on the site- and stereoselectivity. During the course of this investigation, we also unexpectedly discovered that LolT exhibits β-elimination activity with L-cystine and O-acetyl-L-serine, confirming our previous hypothesis based on structural and phylogenetic analysis that LolT, a Cα-C bond forming enzyme, is evolved from a primordial Cβ-S lyase family. Overall, our study demonstrates that LolT is an extremely versatile biocatalyst, and can be used for not only heterocyclic quaternary amino acid biosynthesis, but also biocatalytic amino acid deuteration.
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