Evidence map›Paper›PMID 37775654›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production.

Tejaswini Ramakrishna Hegde, Ogechi Okocha Rufus, Joongoo Lee, Seok Hoon Hong

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Tejaswini Ramakrishna HegdeDepartment of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA.
Ogechi Okocha RufusDepartment of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA. ookocharufus@hawk.iit.edu.
Joongoo LeeDepartment of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea.
Seok Hoon HongDepartment of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA.
Illinois Institute of Technology · USPohang University of Science and Technology · KR

Funding

Engineering Colicins to Control Antibiotic-Tolerant BacteriaR15AI130988 · NIAID · ILLINOIS INSTITUTE OF TECHNOLOGY · PI HONG, SEOK HOON · 2018 to 2018
$437k
NIAID NIH HHS R15 AI130988
6 · The paper itself

Abstract

Cell-free protein synthesis provides a flexible platform for the production of difficult-to-express proteins, because maintaining cell viability is unnecessary. The antimicrobial proteins known as bacteriocins have great potential for development as antibiotic alternatives. Here, we describe detailed protocols for producing and characterizing colicins-antimicrobial proteins that are produced by Escherichia coli hosts and inactivate nonhost E. coli strains. Active colicins can be produced with lysates containing molecular chaperones and coproduction of immunity proteins in cell-free protein synthesis reactions.

Indexed as

Anti-Infective AgentsBacteriocinsColicinsAnti-Bacterial AgentsEscherichia coliAnti-Bacterial AgentsAnti-Infective AgentsBacteriocinsColicinsAntimicrobial proteinsCell-free protein synthesisChaperonesColicinsInhibition Zones

Identifiers

PMID37775654
OpenAlexW4387162521

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.