ArticleMicrobial cell factories2023
Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale.
Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Recent Advances in Sustainable Yeast-Based Single-Cell Protein Production from Renewable Substrates Using Engineering Approaches.Journal of microbiology and biotechnology · 2026Review
- Random mutagenesis platform for accelerated genome evolution (RAMPAGE) ofEngineering microbiology · 2026Article
- Toward Rational Design of Precision-Fermented Milk Proteins: Integrating Cross-Species Selection, Post-Translational Modification, and AI Optimization.Comprehensive reviews in food science and food safety · 2026Review
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Evolutionary Adaptation and Targeted Metabolic Engineering Synergistically Improve Methanol-Derived Single-Cell Protein Production in Pichia pastoris.Biotechnology journal · 2026Article
- Tri-valorization of methanol in a single bioreactor: co-production of enzyme, chemical, and single-cell protein using engineered Pichia pastoris (Komagataella phaffii).Bioresources and bioprocessing · 2026Article
- Nutritional Value and Food Safety Assessment of Single-Cell Protein Derived fromFoods (Basel, Switzerland) · 2026Article
- Meta-analysis of fruit waste-derived single-cell protein for programmable nutrition via synthetic biology in sustainable food systems.NPJ science of food · 2026Article
- The functional evaluation of Pichia pastoris hydrolysate as a protein source partial replacement of soybean meal in diets of growing-finishing pigs.Porcine health management · 2026Article
- Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects.Synthetic and systems biotechnology · 2026Review
- One-carbon-derived bioactive peptides improve reproductive performance via regulating placental nutrient transport and offspring glycolipid metabolism.NPJ science of food · 2026Article
- Robust Pichia kudriavzevii for open production of methylotrophic protein.Applied microbiology and biotechnology · 2026Article
- Yeast Efficiently Utilizes Ribosomal RNA-Derived Oligonucleotides as Bioavailable Nutrient Sources.Foods (Basel, Switzerland) · 2026Article
- The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5.Microbial biotechnology · 2025Article
- Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.Nature communications · 2025Article
- Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.Journal of agricultural and food chemistry · 2025Article
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.FEMS yeast research · 2025Review
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- Unforeseen current and future benefits of uncommon yeast: the Metschnikowia genus.Applied microbiology and biotechnology · 2024Review
- Metabolic engineering for single-cell protein production from renewable feedstocks and its applications.Advanced biotechnology · 2024Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMethanol, synthesized from CO
resultsIn the present work, adaptive laboratory evolution (ALE) has been employed to overcome the low methanol utilization efficiency and intolerance to a higher temperature of 33 °C in P. pastoris, associated with reduced carbon loss due to the lessened detoxification of intracellular formaldehyde through the dissimilation pathway and cell wall rearrangement to temperature stress resistance following long-term evolution as revealed by transcriptomic and phenotypic analysis. By strengthening nitrogen metabolism and impairing cell wall synthesis, metabolic engineering further increased protein content. Finally, the engineered strain via multi-strategy produced high levels of SCP from methanol in a pilot-scale fed-batch culture at 33 °C with a biomass of 63.37 g DCW/L, methanol conversion rate of 0.43 g DCW/g, and protein content of 0.506 g/g DCW. SCP obtained from P. pastoris contains a higher percentage of protein compared to conventional foods like soy, fish, meat, whole milk, and is a source of essential amino acids, including methionine, lysine, and branched-chain amino acids (BCAAs: valine, isoleucine, leucine).
conclusionsThis study clarified the unique mechanism of P. pastoris for efficient methanol utilization, higher temperature resistance, and high protein synthesis, providing a P. pastoris cell factory for SCP production with environmental, economic, and nutritional benefits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.