Evidence map›Paper›PMID 37768976›Full record

ArticlePloS one2023

Molecular characterization of juvenile fish from the Amazon estuary using DNA barcoding approach.

Ítalo Lutz, Thais Martins, Felipe Araújo, Charles Ferreira, Paula Santana, Josy Miranda, Suane Matos, Jefferson Sousa, Luciano Pereira, Bianca Bentes and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ítalo LutzLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Thais MartinsLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Felipe AraújoCoordenação de Zoologia, Museu Paraense Emílio Goeldi, Belém, Pará, Brazil.ORCID 0000-0003-2668-7843
Charles FerreiraLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Paula SantanaLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Josy MirandaLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Suane MatosLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Jefferson SousaLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Luciano PereiraNúcleo de Ecologia Aquática e Pesca da Amazônia, Universidade Federal do Pará, Belém, Pará, Brazil.
Bianca BentesNúcleo de Ecologia Aquática e Pesca da Amazônia, Universidade Federal do Pará, Belém, Pará, Brazil.
Raimundo da SilvaLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Ivana VenezaUniversidade Federal do Oeste do Pará, Monte Alegre, Pará, Brazil.
Iracilda SampaioLaboratório de Evolução, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Marcelo VallinotoLaboratório de Evolução, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
Grazielle Evangelista GomesLaboratório de Genética Aplicada, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.ORCID 0000-0002-3003-7272

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The efficiency of the DNA barcoding relies on sequencing fragment of the Cytochrome C Subunit I (COI) gene, which has been claimed as a tool to biodiversity identification from distinct groups. Accordingly, the goal of this study was to identify juvenile fish species along an estuary of Caeté River in the Brazilian Blue Amazon based on. For this purpose, we applied the DNA barcoding and discuss this approach as a tool for discrimination of species in early ontogenetic stages. A 500-bp fragment was obtained from 74 individuals, belonging to 23 species, 20 genera, 13 families and seven orders. About 70% of the 46 haplotypes revealed congruence between morphological and molecular species identification, while 8% of them failed in identification of taxa and 22% demonstrated morphological misidentification. These results proved that COI fragments were effective to diagnose fish species at early life stages, allowing identifying all samples to a species-specific status, except for some taxa whose COI sequences remain unavailable in public databases. Therefore, we recommend the incorporation of DNA barcoding to provide additional support to traditional identification, especially in morphologically controversial groups. In addition, periodic updates and comparative analyses in public COI datasets are encouraged.

Indexed as

DNA Barcoding, TaxonomicEstuariesAnimalsDNAElectron Transport Complex IVFishesHumansPhylogenyDNAElectron Transport Complex IV

Identifiers

PMID37768976
PMCPMC10538654

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.