ArticlePloS one2023
Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Genetic diversity of Cimex hemipterus and first report of Wolbachia supergroup T in Tehran, Iran: insights from COI and 16 S rRNA analysis.Scientific reports · 2026Article
- Detection and Genetic Diversity of Heritable Bacterial Symbionts in Human Lice Based on 16S-rRNA Gene.Environmental microbiology reports · 2026Article
- Molecular identification and characterization ofCurrent research in parasitology & vector-borne diseases · 2026Article
- Impact of rising temperatures on the bacterial communities of Aphaenogaster ants.Biology open · 2025Article
- Invasive indoor pests under the microbiological lens: bacterial and viral diversity from local to global scales in bed bugs and cockroaches.Current opinion in insect science · 2025Review
- Prevalence of Heritable Symbionts in Parisian Bedbugs (Hemiptera: Cimicidae).Environmental microbiology reports · 2025Article
- Exploring genetic diversity and phylogenetic connections of tropical bed bugs, Cimex hemipterus (F.) infestation in Indonesia.PloS one · 2025Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis study aimed to investigate the genetic diversity of Wolbachia in field-caught bed bug species in Paris areas.
methodsThe bed bug specimens were captured from various infested localities in Paris and surrounding cities. They belonged to diverse life stages, including egg, nymph, and adult. They were then identified using morphological and molecular approaches. Furthermore, Wolbachia was detected, and its genetic diversity was investigated by conventional PCR of 16S-rRNA and Wolbachia surface protein (wsp) genes.
resultsA total of 256 bed bug specimens belonging to various life stages [adult (183 specimens), nymph (48), and egg (25)] were captured from seven private apartments, five social apartments, three houses, two immigrant residences, and one retirement home situated in 10 districts of Paris and 8 surrounding cities. They were identified as Cimex lectularius (237 specimens) and C. hemipterus (19) using morphological and molecular approaches. The presence and diversity of Wolbachia were ascertained by targeting 16S-rRNA and wsp genes. Based on molecular analysis, 182 and 148 out of 256 processed specimens were positive by amplifying 16S-rRNA and wsp fragments, respectively. The inferred phylogenetic analysis with 16S-rRNA and wsp sequences displayed monophyletic Wolbachia strains clustering each one in three populations. The median-joining network, including the Wolbachia 16S-rRNA and wsp sequences of C. lectularius and C. hemipterous specimens, indicated a significant genetic differentiation among these populations in Paris areas which was consent with Neighbor-Joining analyses. A phylogenetic analysis of our heterogenic Wolbachia sequences with those reported from other arthropod species confirmed their belonging to supergroup F. Moreover, no difference between Wolbachia sequences from eggs, nymphs, and adults belonging to the same clade and between Wolbachia sequences of C. lectularius and C. hemipterus were observed after sequence alignment. Furthermore, no significant correlation was found between multiple geographical locations (or accomodation type) where bed bugs were collected and the genetic diversity of Wolbachia.
conclusionsWe highlight a significant heterogeneity within Wolbachia symbionts detected in C. lectularius and C. hemipterus. No correlation between Wolbachia species and bed bug species (C. lectularius versus C. hemipterus), physiological stages (egg, nymph, and adult), and sampling location was recorded in this study.
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